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Common Use Equipment Room

Common Use Equipment Protocol
Training Videos
Equipment Calendar Instructions

The Genomics Core maintains some select equipment available for public use:

Located in Room S-10 Plant Biology:

  • Invitrogen Qubit fluorometer for DNA/RNA quantitation
  • Covaris M220 sonicator for DNA fragmentation
  • FLUOstar OPTIMA microplate reader for high throughput DNA/RNA quantitation

Due to the COVID-19 pandemic, the Biomek 3000 liquid handler robot in Room S-18 Plant Biology is currently unavailable for use.


Common use equipment protocol

Access to our common use equipment room (Room S-10 Plant Biology Laboratories) is restricted at this time due to the COVID-19 pandemic. The following procedures must be followed for researchers to access the common use equipment room.

1. In order to access the room, a reservation must be made at least 12 hours in advance by using our PLB.RoomS10.Equipment Calendar.  In order to observe safe social distancing policies, the reservation calendars for the three instruments in this room have been combined into a single reservation calendar.  Reservations must not exceed 3 hours and work must be completed between the hours of 8 AM and 5 PM Monday through Friday (excluding holidays).  See the Equipment Calendar Instructions to make a reservation.

2. An email must be sent to gtsf@msu.edu indicating the date and time of the new reservation, the instrument that will be used and the number of samples that will be processed. This will give the Genomics Core staff time to ensure that the appropriate reagents and disposables will be available for the researcher. It is necessary to indicate in the email if you do not have keycard access to the Plant Biology Laboratories. If the researcher does not have keycard access, a Genomics Core lab member will provide access to the building.

  • If you will be using the Qubit, how many samples will be processed, and what type of assay will be used (DNA high sensitivity, DNA broad range, RNA high sensitivity, RNA broad range)? 
  • If you will be using the Covaris Sonicator, how many samples will be processed and which sonication tube will be used (microTUBE for 150 bp to 1.5 kbp fragments or miniTUBE for 2, 3 and 5 kbp fragments)?

3. If it is necessary for a Genomics Core lab member to provide access to the Plant Biology Laboratories building, a Genomics Core lab member will meet you at the south entrance to the building. Before arriving to the Plant Biology Building, the researcher must complete the sign in/sign out online form.

4. A copy of the Plant Biology Laboratories safety procedures and policies will be sent to the researcher before the scheduled reservation time and must be read prior to the reservation time:

5. Only a single person will be allowed to work in the room at one time.

6. Visitors to the Plant Biology Laboratories building must wear PPE including face mask and eye protection (eye glasses or safety glasses). In the common use equipment room, researchers must wear gloves, which will be provided.

7. Hand sanitizer will be available within the common use equipment room.

8. Researchers must clean the equipment before and after use. A supply of 70% ethanol and paper towels will be available in the common use equipment room.

9. Researchers whose labs are outside of the Plant Biology Laboratories Building must complete the online form indicating that they are leaving the Common Use Equipment Room and the building by using the sign in/sign out form.

10. In-person training will not be available for the common use equipment. Researchers should download, print out and read the manuals for the Qubit 1.0 or the Covaris M220 Sonicator.  A training video for using the Qubit 1.0 in the Common Use Equipment Room is now available.  An online manual for the FLUOstar Optima is not available. Write to gtsf@msu.edu if you need training for the FLUOstar Optima. A training video for the Covaris M220 Sonicator will be made available in the near future.


Training Videos

Qubit Training Video


 

Covaris Training Video

Coming Soon!

 


Equipment calendar Instructions

Reservations must not exceed 3 hours and work must be completed between the hours of 8 AM and 5 PM Monday through Friday.

In order to observe safe social distancing policies the common use equipment room will only be available to a single user at a time. In order to schedule bookings without conflict a resource calendar has been set up using the University's MS Office 365 Outlook Calendar. An MSU NetID is required to access these calendars. Configuration and scheduling reservations using this calendar differs depending on whether you access it via an MS Outlook desktop application or the Outlook Web Application. You may download detailed instructions specific for the method you use to access Office 365:

After submitting an appointment request you will receive an email (and possibly a Calendar notification) either confirming or declining your request. If your request is declined a reason will be given in the email. If you do not receive an automated reply within a few minutes of making your request then your reservation was NOT made. If this happens or you have any questions please contact the Genomics Core (gtsf@msu.edu).

After receiving confirmation of your appointment follow the Common Use Equipment Protocol above.