QPCR Services

ABI Quant Studio 7Flex
The ABI QuantStudio 7 Flex PCR system is a robust platform allowing the broadest range of PCR applications for real-time quantitation of gene expression and for detection of single nucleotide polymorphisms (SNPs). PCR quantitation can be carried out using either the TaqMan or SYBR green chemistries and the system offers 384-well capacity and robotic loading for high throughput capability.

Submission Guidelines
•    Assembled reactions can be submitted in approved 384-well plates (10ul reaction volume) ready to run or in 96 well plates (DOT Scientific #800-PCR or equivalent; 15ul reaction volume) and we will transfer up to four 96-well plates into each 384 well-plate.
•    Standard cycling parameters will be used for all samples unless otherwise stated. 
•    Submit plate(s) before 4 pm Monday through Friday to obtain data the next business morning.
•    Analysis software is available at our facility (S-18 Plant Biology Building).

 

WaferGen SmartChip Real-Time PCR System
The SmartChip Real-Time PCR System is a next-generation, real-time PCR system that combines the high throughput nature of microarrays with the sensitivity, precision and dynamic range of quantitative real-time PCR. It is an open platform with the ability to customize gene expression and genotyping assays according to your needs and is compatible with both probe based and SYBR chemistries.

Central to the system is the 5,184 well SmartChip in which 100nl reactions can be assembled utilizing the Multisample Nanodispenser in one of 14 preconfigured formats.

The chip is then loaded into the SmartChip cycler for thermocycling and real time signal detection.

For more information please contact Emily Crisovan.