The RTSF Genomics Core at Michigan State University offers a host of accessory services we provide to assist in QC for our NGS services.
NGS Library Size Selection
Isolating DNA fragments of a defined size range is important for many applications, NGS library construction in particular. The RTSF Genomics Core can now perform automated size selection accurately using the Sage Science Blue Pippin. This platform uses gel cassettes to provide a full range of size selection options between 90 bp to 50 kb. For additional information visit http://www.sagescience.com/products/bluepippin/.
Preparing high quality libraries for sequencing is paramount for obtaining good data and the majority of protocols require fragmentation of the nucleic acid material to a specific size range. Mis-sizing of the libraries can result in inaccurate quantification, poor cluster density and subsequently low quality data.
The RTSF Genomics Core provides shearing services for all NGS library types using the Covaris S2 Adaptive Focused Acoustic Disruptor. This platform enables improved preparation of small biological samples up to 100ul in volume. Applications include DNA and chromatin shearing, tissue disruption and homogenization, and cell lysis among others.
Library construction is the process of taking nucleic acid from its original state to a "library" that can be run on a sequencing platform. Preparing high quality libraries is important for obtaining good data and the RTSF Genomics Core checks each sample before and after the library construction process to ensure it is of high quality. Concentration, size and quality can be assessed using one of our microfluidics based platforms.
The Agilent Bioanalyzer system provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform, providing high quality digital data.
The Caliper Labchip GX
The LabChip GX utilizes PerkinElmer's innovative microfluidics technology to perform reproducible, high-resolution, eletrophoretic separations. Whether analyzing RNA integrity for better gene expression data or assessing PCR fragments for resequencing applications, the GX provides automated walkaway sample QC and is ideally suited for batches of 36+ samples.
Please contact Emily Crisovan for more information on Services.