Pricing

Illumina Next Generation Sequencing

 

Illumina Library Preparation 

Standard library preparation is performed with Illumina TruSeq kits. Alternatives may be available for low-input or low-quality starting material. Please contact the Genomics Core for more information.
Kit Type Use 1-16 Libraries 17-32 Libraries 33+ Libraries
TruSeq Nano DNA Shotgun DNA Library Preparation $109.00 $92.00 $86.00
TruSeq Stranded mRNA Sequencing of poly-adenylated mRNA 1 $140.00 $122.00 $115.00
TruSeq Stranded Total RNA Sequencing of RNA with RiboZero rRNA depletion2 $207.00 $188.00 $182.00
TruSeq Small RNA Sequencing of miRNA $176.00 $168.00 $165.00
1Appropriate for high quality, eukaryotic RNA samples
2May be used for bacterial RNA, low-quality (e.g. degraded) eukaryotic RNA, or when you wish to capture ncRNA along with mRNA

 

Other Library Services

 

Service Use Per Library
16S V4 or 16S V3V4 amplicon library 1 Amplicon library preparation of the 16S V4 or V3V4 region. $8 per sample
Amplicon barcoding 1 Barcoding of primary PCR product. $8 per sample
Nextera Mate Pair Sequencing libraries with large span distance between paired reads, user specified in range from 2-10kbps $550.00 First library from an input DNA sample; one size specified
    $50.00 For each additional size requested from input DNA (up to two additional)
QC of submitter prepared library Qubit dsDNA, Caliper LabChipGX and Kapa qPCR assays $20.00/library or pool
1See our sample requirements page for more information.

 

HiSeq 4000

HiSeq 4000 services are purchased per lane. Output per lane: Illumina's Specs: 264-312 million clusters passing filter (CPF); Genomics Core's frequent output: 320-375 million CPF; Genomics Core's average ~350 CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluster will generate one forward read and one reverse read.

 

Kit Type/Size Sequence Format3 Per Lane Approximate Output (Gbp)
50 cycle 1 x 50bp single end $911 17.5
300 cycle 2 x 150bp paired end $2,347 105
150 cycle4 2 x 75bp paired end $1,686 52.5
3Other sequencing formats may be possible but would require the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.
4Requires the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.

 

MiSeq

 

Kit Type/Size Sequence Format Per Lane Expected Output(GBp)4 Reads Output (M)
v2 Standard 50 cycle 1 x 50bp single end $909 0.6-0.75 12-15
v2 Standard 300 cycle 2 x 150bp paired end $1,200 3.6-4.5 12-15
v2 Standard 500 cycle 2 x 250bp paired end $1,315 6.0-7.5 12-15
v2 Micro 300 cycle 2 x 150bp paired end $610 1.2 4
v2 Nano 300 cycle 2 x 150bp paired end $465 0.3 1
v2 Nano 500 cycle 2 x 250bp paired end $581 0.5 1
v3 150 cycle 2 x 75bp paired OR 1 x 150bp single end $1,017 3.3-3.8 22-25
v3 600 cycle 2 x 300bp paired end $1,799 13-15

22-25

4When sequencing low diversity libraries, e.g. amplicon libraries for metagenomics, output will be reduced by ~20%.

 

Sanger Sequencing and Genotyping

Service Description Per Sample      
Cell ID Cell line authentication with Promega GenePrint $35.00      
Fragment Analysis AFLP DNA fingerprinting $1.50      
Short Tandem Repeat(STR)   $1.50      
    Standard Reaction 1st Tier5 Genomic6 GTP7
Low throughput (recommended for ≤8 samples)1,2 Submit in individual tubes $7.00 $8.00 $12.00 $13.00
High throughput (9-16 samples)3,4 Submit in 96 well plates (arrange by column) $5.00 $6.00 $12.00 $13.00
High throughput (≥17 samples) Submit in 96 well plates (arrange by column) $3.50 $4.50 $12.00 $13.00
1Low throughput submission: DNA in 12µl, you may add a custom primer or RTSF will add standard primers
2Submission of more than 8 samples in individual tubes will still be charged at the Low throughput price
3High throughput submission: DNA in 6µl, you may add a custom primer or RTSF will add standard primers
4Submission of 9-16 samples may be in 8-tube strips
5DMSO added to reaction, appropriate for high GC DNA or plasmids > 10kbp
6Samples which are high molecular weight genomic DNA
7DMSO, betaine, and dGTPS added to reaction, appropriate for samples with hairpins or significant secondary structure

 

qPCR Services

 

QuantStudio 7 Flex qPCR Services

Users submit completely assembled reactions in 96 or 384 well plates.

 

Service Submission Per 7900 Run
Absolute Quantitation1 1 x 96 well plate $45.00
  2 x 96 well plate $60.00
  3 x 96 well plate $75.00
  4 x 96 well plate $90.00
  1 x 384 well plate $40.00
Dissociation Curve Cost in addition to Absolute Quantitation above $8.50
1Price includes transfer from 96 to 384 well plates and processing on QuantStudio 7 Flex.

 

qPCR Supplies Available for Purchase

 

Item Price
384 well optical plate $5.00 each
Optical sealing film $1.50 each

Other Expression Analysis Services

Service Description Price
SmartChip RT-qPCR (Takarabio/Wafergen) Flexible, very high-throughput RT-qPCR with 5,184 qPCR reactions $430.00 per basic run (user supplies prepared 384-well plates)
    $490.00 per basic run (Genomics Core assembles 384-well assay and sample plates)
NanoString nCounter Direct DNA/RNA counting with predefined panels mRNA $260.00/12 sample cassette
    miRNA $370.00/12 sample cassette
    PlexSet $345.00/96 samples in 12 well cassette
    CNV $435.00/12 sample cassette

 

 

Sample QC and Other

Service Description Price
Qubit1 Fluorometric DNA/RNA quantitation $0.60/sample
Agilent 2100 Bioanalyzer1 - DNA/RNA microcapilliary based sizing and quality assay DNA 1000 Chip - up to 12 samples/chip $35.00/chip
  DNA High Sensitivity Chip - up to 11 samples/chip $55.00/chip
  RNA Pico Chip - up to 11 samples/chip $35.00/chip
Covaris AFA DNA shearing1 Acoustic shearing of dsDNA $8.50/sample (includes cost of Covaris tube)
1These are self-service equipment. All users must be trained by Genomics Core staff prior to use. To schedule a training session, please contact

 

 

 

All payment methods, besides MSU accounts, will be subject to a 2% University Administrative Fee.