Pricing

All payment methods, besides MSU accounts, will be subject to a 2% University Administrative Fee.


Oxford Nanopore Library Preparation

Oxford Nanopore Flow Cells

Illumina-Compatible Library Preparation

HiSeq 4000

NextSeq 500

MiSeq

Sanger Sequencing

Genotyping/Fragment Analysis

QuantStudio 7 Flex qPCR

SmartChip RT-qPCR

NanoString

TapeStation and Bioanalyzer Analysis

Qubit Fluorometric Quantitation

Covaris Sonicator

 

Oxford Nanopore Library Preparation

Nanopore library preparations that are offered by the Genomics Core are listed below. Descriptions of these library kits can be found on the Nanopore Sequencing Technology page. One library preparation is required for each sequencing flow cell, and flow cell prices are listed below. Sequencing yields with current Nanopore library kits and the current flow cells is highly dependent on sample quality. The Nanopore Sequencing Technology page has some guidance on yields. Also see the Nanopore Sample Requirements page for information about submission requirements. Please contact the Genomics Core if you have questions.

Library Kit Name Nanopore Kit ID Sequencing Target Price Per Library*
1D Ligation Sequencing Kit LSK109 Genomic DNA $410
1D Ligation Sequencing Kit (without tagmentation) LSK108 Long Amplicons and User-Barcoded Amplicons $420
Rapid Barcoding Kit
(up to 12 samples)
RBK004 Multiplexed Genomic DNA (small genomes) $320
Direct RNA Sequencing Kit RNA002 Full Length RNA $330
cDNA-PCR Sequencing Kit (no barcoding) PCS109 Full Length cDNA $475
cDNA-PCR Sequencing Kit with barcoding (up to 12 samples) PCS109, PBK004 Multiplexing Full Length cDNA for a single flow cell $490 + $60/barcode
Direct cDNA Sequencing Kit DCS109 Full Length cDNA $470

*When two or more libraries of the same type are submitted, a 10% discount will be applied to those library preparations.

 

Oxford Nanopore Flow Cells

Currently, the Genomics Core only offers the SpotON Flow Cell Rev D (FLO-MIN106D) flow cells for running on our GridION instrument. As new flow cells are released by Oxford Nanopore, additional flow cell designs may become available at the Genomics Core. The SpotON Flow Cell Rev D is not appropriate for 1D2 libraries. Researchers may submit their own libraries for sequencing in the Genomics Core, but it is necessary to contact the Genomics Core before submission in order to coordinate with us. One library preparation is required for each sequencing flow cell, and library preparation prices are listed above. Flow cells are not available for sale directly to researchers. Please contact the Genomics Core if you have questions.

Flow Cell Name Nanopore ID Sequencing Instrument Price
SpotON Flow Cell Rev D (R9.4.1) FLO‑MIN106D GridION $665
SpotON Flow Cell Rev D (R10)* FLO‑MIN110 GridION $665

*Please contact the Genomics Core (gtsf@msu.edu) to discuss availability of R10 flow cells.

 

Illumina-Compatible Library Preparation 

Standard library preparation is performed using the following library preparation kits. Alternatives may be available for low-input or low-quality starting material. Please contact the Genomics Core for more information.

1Appropriate for high quality, eukaryotic RNA samples.

2May be used for low-quality (e.g. degraded) eukaryotic RNA, or when you wish to capture ncRNA along with mRNA. These kits are species specific, please contact the Genomics Core for more information about pricing and availability.

Library Kit Name Use Price Per Library (1-16 Libraries) Price Per Library (17-32 Libraries) Price Per Library (≥33 Libraries)
Illumina TruSeq Nano DNA Shotgun DNA Library Preparation $109 $92 $86
TakaraBio SMARTer ThruPLEX DNA‑Seq Low Input DNA Library Preparation or ChIP-seq Library Preparation $109 $92 $86
Illumina TruSeq Stranded mRNA Library1 Library preparation of poly-adenylated mRNA $140 $122 $115
Illumina TruSeq Stranded Total RNA Library with Ribo‑Zero Depletion2 Library preparation of ribosomal RNA depleted total RNA Contact Genomics Core for availability and pricing.
Illumina TruSeq Small RNA Library Library preparation of microRNA from 22 - 30 nt.  Libraries can also be made from small RNAs > 30 nt, contact the Genomics Core to discuss project. $176 $168 $165

Lexogen QuantSeq Library Preparation

3UMIs are included in the library prep so that PCR duplicates can be identified and eliminated before read analysis.  Contact the Genomics Core if you do not want the UMIs to be included in your library prep.

Library Kit Name Use Price Per Library (up to 47 samples) Price Per Library (48-71 samples) Price Per Library (≥72 samples)
Lexogen QuantSeq 3' mRNA‑Seq Library Prep Kit FWD with Unique Molecular Indentifiers (UMIs) Quant-seq generates highly strand-specific libraries of reads from the 3' end of each mRNA in your samples. Only one read is created from each mRNA, and many fewer reads are required for gene expression analysis. $62 $56 $53

Swift Methyl-Seq Library Preparation

 

Library Kit Name Use Price Per Library
Swift BioSciences Accel‑NGS Methyl‑Seq DNA Library Methyl‑Seq DNA Library Preparation. Whole Genome Bisulfite Sequencing (WGBS) and Reduced Representation Bisulfite Sequencing (RRBS) are possible. Contact the Genomics Core to discuss RRBS. Bisulfite conversion is performed using Zymo EZ DNA Methylation- Gold Kit. $178

Other Library Services

4For amplicon barcoding, a two-step PCR approach is used.  The user-prepared PCR uses target-specific primers with tags on the 5' ends that allow the addition of barcodes during the secondary PCR step.  Our sample requirements page describes the sequences that must be added to your target-specific primers in order to allow barcoding during the secondary PCR step.

 

Service Use Price per Library
16S V4 Amplicon Library Amplicon library preparation of the 16S V4 region. $8
16S V3V4 Amplicon Library Amplicon library preparation of the 16S V3V4 region. $8
Amplicon barcoding4 Barcoding of user-prepared primary PCR products such as ITS or other regions of interest. $8
QC of submitter prepared library or pool Qubit dsDNA, Agilent 4200 TapeStation or 2100 Bioanalyzer, and Kapa qPCR assays $20/library or pool

HiSeq 4000

HiSeq 4000 services are purchased per lane. Output per lane: Illumina's Specs: 264-312 million clusters passing filter (CPF); Genomics Core's frequent output: 320-375 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluster will generate one forward read and one reverse read.

5Other sequencing formats may be possible but would require the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.

6Requires the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.

Kit Type/Size Sequence Format5 Per Lane Approximate
Output (Gbp)
50 cycle 1 x 50bp single end $1,002 17.5
300 cycle 2 x 150bp paired end $2,661 105
150 cycle 2 x 75bp paired end $1,8746 52.5

 

NextSeq 500

Output per flow cell: Illumina specs: High output flow cells will generate up to 400 million clusters passing filter (CPF) and Mid output flow cells will generate up to 130 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluster will generate one forward read and one reverse read.

Kit Type/Size Sequence Format Per Run Approximate
Output (Gbp)
High Output 75 cycle 1 x 75bp single end or
2 x 35bp paired end
$1,712 30
High Output 150 cycle 1 x 150bp single end or
2 x 75bp paired end
$3,194 60
High Output 300 cycle 2 x 150bp paired end $5,129 120
Mid Output 150 cycle

1 x 150bp single end or
2 x 75bp paired end

$1,384 20
Mid Output 300 cycle 2 x 150bp paired end $2,239 39

 

 

MiSeq

7When sequencing low diversity libraries, e.g. amplicon libraries for metagenomics, output will be reduced by ~20%.

Kit Type/Size Sequence Format Per Lane Approximate
Output (Gbp)7
Reads Output (M)
v2 Standard 50 cycle 1 x 50bp single end $954 0.6-0.75 12-15
v2 Standard 300 cycle 2 x 150bp paired end $1,264 3.6-4.5 12-15
v2 Standard 500 cycle 2 x 250bp paired end $1,376 6.0-7.5 12-15
v2 Micro 300 cycle 2 x 150bp paired end $634 1.2 4
v2 Nano 300 cycle 2 x 150bp paired end $484 0.3 1
v2 Nano 500 cycle 2 x 250bp paired end $601 0.5 1
v3 150 cycle 2 x 75bp paired or
1 x 150bp single end
$1,072 3.3-3.8 22-25
v3 600 cycle 2 x 300bp paired end $1,894 13-15

22-25

 

Sanger Sequencing

8Low throughput submission: DNA in 12µl, you may add a custom primer or Genomics Core will add standard primer.

9Submission of more than 8 samples in individual tubes will be charged the low throughput price.

10High throughput submission: DNA in 6µl, you may add a custom primer or Genomics Core will add standard primer.

11DMSO added to reaction, appropriate for high GC DNA or plasmids > 10 kbp.

12Samples which are high molecular weight genomic DNA

13DMSO, betaine, and dGTPS added to reaction, appropriate for samples with hairpins or significant secondary structure.

Service Description Standard Reaction 1st Tier11 Genomic12 GTP13
Sanger Sequencing - Low throughput (recommended for ≤8 samples)8,9 Submit in individual tubes $7/sample $8/sample $12/sample $13/sample
Sanger Sequencing - High throughput (9-16 samples)9,10 Submit in 96 well plates (arrange by column) or 8-strip PCR tubes $5/sample $6/sample $12/sample $13/sample
Sanger Sequencing - High throughput (≥17 samples)9,10 Submit in 96 well plates (arrange by column) $3.50/sample $4.50/sample $12/sample $13/sample

 

Genotyping/Fragment Analysis

Service Description Per Sample
Cell ID Cell line authentication with Promega GenePrint 10 System $35
Fragment Analysis AFLP DNA fingerprinting or Short Tandem Repeat (STR) $1.50

 

 

QuantStudio 7 Flex qPCR Services

Users submit completely assembled reactions in 96- or 384-well plates.

14Price includes transfer from 96-well plate(s) to 384-well plate and processing on QuantStudio 7 Flex.

Service Submission Per Run
Absolute Quantitation14 1 x 96 well plate $45
  2 x 96 well plate $60
  3 x 96 well plate $75
  4 x 96 well plate $90
  1 x 384 well plate $40
Dissociation Curve Cost in addition to Absolute Quantitation above $8.50

qPCR Supplies Available for Purchase

Item Price
384 well optical plate $5/each
Optical sealing film $2/each

 

 

Other Expression Analysis Services

15Price does not include reagents from NanoString. The listed prices are for sample processing only.

Service Description Price
SmartChip RT-qPCR (Takarabio/Wafergen) Flexible, very high-throughput RT-qPCR with 5,184 qPCR reactions $430 per basic run (user supplies prepared 384-well plates)
    $490 per basic run (Genomics Core assembles 384-well assay and sample plates)
NanoString nCounter15 mRNA assays with Gene Expression Panels, Custom CodeSets or Elements TagSets mRNA $260/12 sample cassette
  miRNA assays with NanoString miRNA Panels miRNA $370/12 sample cassette
  Multiplexed mRNA assays with nCounter PlexSets PlexSet $345/96 samples in 12 well cassette

 

TapeStation & Bioanalyzer Analysis Services

The Genomics Core has an Agilent 4200 TapeStation and an Agilent 2100 Bioanalyzer.  Both instruments use automated electrophoresis to assess the quality and quantity of DNA and RNA.

All samples will be processed on the TapeStation, unless otherwise requested. The only exception is total RNA that will be used for small RNA or micro RNA library preparation; these samples will be analyzed on the Bioanalyzer because it is more sensitive to small RNA and micro RNA species. 

The 4200 TapeStation analysis is performed on a ScreenTape that has individually addressable lanes, where 1 sample is run per lane.  This allows for analysis of 1 or more samples at a time.  The TapeStation analysis is sold on a per sample basis.  

The 2100 Bioanalyzer analysis is performed on a chip containing 11 wells, which allows for analysis of 1 to 11 samples.  With the 2100 Bioanalyzer, reagents for 11 samples are used even if fewer than 11 samples are analyzed.  Therefore, the Bioanalyzer analysis is sold on a per chip basis (1 to 11 samples). 

Please see the TapeStation & Bioanalyzer Sample Requirements webpage for information regarding sample requirements and submission.

 

TapeStation Analysis Services

Instrument Assay Price
TapeStation

High Sensitivity RNA ScreenTape Assay.

For analysis of total RNA.

$8/sample
TapeStation

High Sensitivity D1000 ScreenTape Assay
(sizing range 35 - 1,000 bp)

or

High Sensitivity D5000 ScreenTape Assay
(sizing range 100 - 5,000 bp).

For analysis of prepared libraries, dsDNA PCR productions, or fragmented DNA. Assay used depends on size of submitted product.

$8.50/sample
TapeStation

Genomic DNA ScreenTape Assay
(sizing range 200 - 60,000 bp)

For analysis of high molecular weight genomic DNA.

$10/sample

 

Bioanalyzer Analysis Services

Instrument Assay Price
Bioanalyzer

RNA 6000 Pico Assay

For analysis of total RNA.

Eleven (11) samples can be analyzed per chip.

$126/chip
Bioanalyzer

High Sensitivity DNA Assay
(sizing range 50 - 7,000 bp)

For analysis of prepared libraries, dsDNA PCR products, or fragmented DNA.

Eleven (11) samples can be analyzed per chip.

$137/chip

 


Qubit Fluorometric Quantification

The Genomics Core has a Qubit 1.0 available for researchers to use for fluorometric quantitation of double-stranded DNA and total RNA.  The Qubit is a self-service instrument.  Users must receive training by Genomics Core staff prior to using this equipment.  Please contact the Genomics Core (gtsf@msu.edu) to schedule a training session.  See the Resource Calendar webpage to make a reservation.

Qubit Kit Name Description Price per sample or standard
Qubit dsDNA BR (Broad Range) Assay

Fluorometric quantitation of double-stranded DNA.

Quantitation range: 2 - 1000 ng.

$1.40
Qubit dsDNA HS (High Sensitivity) Assay

Fluorometric quantitation of double-stranded DNA.

Quantitation range: 0.2 - 100 ng.

$1.40
Qubit RNA BR (Broad Range) Assay

Fluorometric quantitation of total RNA.

Quantitation range: 20 - 1000 ng.

$1.40
Qubit RNA HS (High Sensitivity) Assay

Fluorometric quantitation of total RNA.

Quantitation range: 5 - 100 ng.

$1.40

 

Covaris Sonicator

The Genomics Core has a Covaris m220 focused-ultrasonicator available for researchers to use for fragmentation of DNA.  The Covaris is a self-service instrument.  The Genomics Core stocks small tubes (130 µl tube, p/n 520077) and large tubes (1 ml tube, p/n 520135) for purchase. Users must receive training by Genomics Core staff prior to using this equipment.  Please contact the Genomics Core (gtsf@msu.edu) to schedule a training session.  See the Resource Calendar webpage to make a reservation.  

Description Price
Sample Shearing $1.50/sample
Small Tube (130 µl tube, p/n 520077) $5/tube
Large Tube (1 ml tube, p/n 520135) $7/tube