Pricing

All payment methods, besides MSU accounts, will be subject to a 2% University Administrative Fee.

 

Oxford Nanopore Library Preparation

Nanopore library preparations that are offered by the Genomics Core are listed below. Descriptions of these library kits can be found on the Nanopore Sequencing Technology page. One library preparation is required for each sequencing flow cell, and flow cell prices are listed below. Sequencing yields with current Nanopore library kits and the current flow cells is highly dependent on sample quality. The Nanopore Sequencing Technology page has some guidance on yields. Also see the Nanopore Sample Requirements page for information about submission requirements. Please contact the Genomics Core if you have questions.

Library Kit Name Nanopore Kit ID Sequencing Target Price Per Library*
1D Ligation Sequencing Kit LSK109 Genomic DNA $410
1D Ligation Sequencing Kit (without tagmentation) LSK108 Long Amplicons and User-Barcoded Amplicons $420
Rapid Barcoding Kit (up to 12 samples) RBK004 Multiplexed Genomic DNA (small genomes) $320
Direct RNA Sequencing Kit RNA002 Full Length RNA $330
cDNA-PCR Sequencing Kit (no barcoding) PCS109 Full Length cDNA $475
cDNA-PCR Sequencing Kit with barcoding (up to 12 samples) PCS109, PBK004 Multiplexing Full Length cDNA for a single flow cell $490 + $60/barcode
Direct cDNA Sequencing Kit DCS109 Full Length cDNA $470

*When two or more libraries of the same type are submitted, a 10% discount will be applied to those library preparations.

 

Oxford Nanopore Flow Cells

Currently, the Genomics Core only offers the SpotON Flow Cell Rev D (FLO-MIN106D) flow cells for running on our GridION instrument. As new flow cells are released by Oxford Nanopore, additional flow cell designs may become available at the Genomics Core. The SpotON Flow Cell Rev D is not appropriate for 1D2 libraries. Researchers may submit their own libraries for sequencing in the Genomics Core, but it is necessary to contact the Genomics Core before submission in order to coordinate with us. One library preparation is required for each sequencing flow cell, and library preparation prices are listed above. Flow cells are not available for sale directly to researchers. Please contact the Genomics Core if you have questions.

Flow Cell Name Nanopore ID Sequencing Instrument Price
SpotON Flow Cell Rev D FLO-MIN106D GridION $665

 

Illumina Library Preparation 

Standard library preparation is performed with Illumina TruSeq library preparation kits. Alternatives may be available for low-input or low-quality starting material. Please contact the Genomics Core for more information.

1Appropriate for high quality, eukaryotic RNA samples.

2May be used for bacterial RNA, low-quality (e.g. degraded) eukaryotic RNA, or when you wish to capture ncRNA along with mRNA. These kits are species specific, please contact the Genomics Core for more information.

 

Kit Type Use 1-16 Libraries 17-32 Libraries 33+ Libraries
TruSeq Nano DNA Shotgun DNA Library Preparation $109 $92 $86
TruSeq Stranded mRNA Sequencing of poly-adenylated mRNA 1 $140 $122 $115
TruSeq Stranded Total RNA Sequencing of RNA with Ribosomal RNA depletion2 Contact Genomics Core for availability and pricing.    
TruSeq Small RNA Sequencing of miRNA $176 $168 $165

Other Library Services

3See our sample requirements page for more information.

 

Service Use Per Library
16S V4 or 16S V3V4 amplicon library3 Amplicon library preparation of the 16S V4 or V3V4 region. $8 per sample
Amplicon barcoding3 Barcoding of user-prepared primary PCR products such as ITS or other regions of interest. $8 per sample
QC of submitter prepared library Qubit dsDNA, Agilent 2100 Bioanalyzer or Advanced Analytical's Fragment Analyzer, and Kapa qPCR assays $20/library or pool

HiSeq 4000

HiSeq 4000 services are purchased per lane. Output per lane: Illumina's Specs: 264-312 million clusters passing filter (CPF); Genomics Core's frequent output: 320-375 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluster will generate one forward read and one reverse read.

4Other sequencing formats may be possible but would require the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.

5Requires the purchase of the entire flow cell (8 lanes). Please contact gtsf@msu.edu for more information.

Kit Type/Size Sequence Format4 Per Lane Approximate Output (Gbp)
50 cycle 1 x 50bp single end $1,002 17.5
300 cycle 2 x 150bp paired end $2,661 105
150 cycle5 2 x 75bp paired end $1,874 52.5

 

NextSeq 500

Output per lane: Illumina specs: High output flowcells will generate up to 400 million clusters passing filter (CPF) and Mid output flowcells will generate up to 130 million CPF.  For single end sequencing, each cluster will generate one forward read.  For paired end sequencing, each cluster will generate one forward read and one reverse read.

Kit Type/Size Sequence Format Per Run Approximate Output (Gbp)
High Output 75 cycle 1 x 75bp single end or 2 x 35bp paired end $1,712 30
High Output 150 cycle 1 x 150bp single end or
2 x 75bp paired end
$3,194 60
High Output 300 cycle 2 x 150bp paired end $5,129 120
Mid Output 150 cycle

1 x 150bp single end or
2 x 75bp paired end

$1,384 20
Mid Output 300 cycle 2 x 150bp paired end $2,239 39

 

 

MiSeq

6When sequencing low diversity libraries, e.g. amplicon libraries for metagenomics, output will be reduced by ~20%.

Kit Type/Size Sequence Format Per Lane Expected Output(Gbp)6 Reads Output (M)
v2 Standard 50 cycle 1 x 50bp single end $954 0.6-0.75 12-15
v2 Standard 300 cycle 2 x 150bp paired end $1,264 3.6-4.5 12-15
v2 Standard 500 cycle 2 x 250bp paired end $1,376 6.0-7.5 12-15
v2 Micro 300 cycle 2 x 150bp paired end $634 1.2 4
v2 Nano 300 cycle 2 x 150bp paired end $484 0.3 1
v2 Nano 500 cycle 2 x 250bp paired end $601 0.5 1
v3 150 cycle 2 x 75bp paired OR 1 x 150bp single end $1,072 3.3-3.8 22-25
v3 600 cycle 2 x 300bp paired end $1,894 13-15

22-25

 

Sanger Sequencing

7Low throughput submission: DNA in 12µl, you may add a custom primer or Genomics Core will add standard primer.

8Submission of more than 8 samples in individual tubes will be charged the low throughput price.

9High throughput submission: DNA in 6µl, you may add a custom primer or Genomics Core will add standard primer.

10DMSO added to reaction, appropriate for high GC DNA or plasmids > 10 kbp.

11Samples which are high molecular weight genomic DNA

12DMSO, betaine, and dGTPS added to reaction, appropriate for samples with hairpins or significant secondary structure.

Service Description Standard Reaction 1st Tier10 Genomic11 GTP12
Sanger Sequencing - Low throughput (recommended for ≤8 samples)7,8 Submit in individual tubes $7/sample $8/sample $12/sample $13/sample
Sanger Sequencing - High throughput (9-16 samples)8,9 Submit in 96 well plates (arrange by column) or 8-strip PCR tubes $5/sample $6/sample $12/sample $13/sample
Sanger Sequencing - High throughput (≥17 samples)8,9 Submit in 96 well plates (arrange by column) $3.50/sample $4.50/sample $12/sample $13/sample

 

Genotyping/Fragment Analysis

Service Description Per Sample
Cell ID Cell line authentication with Promega GenePrint 10 System $35
Fragment Analysis AFLP DNA fingerprinting or Short Tandem Repeat (STR) $1.50

 

 

QuantStudio 7 Flex qPCR Services

Users submit completely assembled reactions in 96- or 384-well plates.

13Price includes transfer from 96-well plate(s) to 384-well plate and processing on QuantStudio 7 Flex.

Service Submission Per Run
Absolute Quantitation13 1 x 96 well plate $45
  2 x 96 well plate $60
  3 x 96 well plate $75
  4 x 96 well plate $90
  1 x 384 well plate $40
Dissociation Curve Cost in addition to Absolute Quantitation above $8.50

 

qPCR Supplies Available for Purchase

Item Price
384 well optical plate $5/each
Optical sealing film $1.50/each

 

 

Other Expression Analysis Services

14Price does not include reagents from NanoString. The listed prices are for sample processing only.

Service Description Price
SmartChip RT-qPCR (Takarabio/Wafergen) Flexible, very high-throughput RT-qPCR with 5,184 qPCR reactions $430 per basic run (user supplies prepared 384-well plates)
    $490 per basic run (Genomics Core assembles 384-well assay and sample plates)
NanoString nCounter14 mRNA assays with Gene Expression Panels, Custom CodeSets or Elements TagSets mRNA $260/12 sample cassette
  miRNA assays with NanoString miRNA Panels miRNA $370/12 sample cassette
  Multiplexed mRNA assays with nCounter PlexSets PlexSet $345/96 samples in 12 well cassette

 

Sample QC and Other

15Self-service equipment. All users must be trained by Genomics Core staff prior to use. To schedule a training session, please email gtsf@msu.edu.

16Fee-for-service instruments.

17See our RNA/DNA Sample Analysis page for more information.

Service Description Price
Qubit15 Fluorometric DNA/RNA quantitation $1.40/sample or standard
Covaris AFA DNA shearing - Acoustic shearing of dsDNA15 Small tube (130 ul tube, p/n 520077) $5/tube
  Large tube (1 ml tube, p/n 520135) $7/tube
  Shearing $1.50/sample
Agilent 2100 Bioanalyzer or Advanced Analytical Fragment Analyzer Analysis - Both instruments use capillary electrophoresis for sizing nucleic acids16 11 RNA or DNA samples/run17 $56 for the first run, additional runs are $50 each