RNA/DNA Sample Analysis: Bioanalyzer & Fragment Analyzer

 

The Genomics Core has many Agilent 2100 Bioanalyzer and Advanced Analytical Fragment Analyzer kits available for sample analysis of RNA, submitter prepared libraries or dsDNA PCR products, and high molecular weight genomic DNA. Genomics Core Staff will select the appropriate kit based on details provided by the researcher at time of submission. 

The table below describes the specifications for submitting samples to the Genomics Core for analysis on the Agilent 2100 Bioanalyzer or the Advanced Analytical Fragment Analyzer.  Both instruments use capillary electrophoresis for sizing nucleic acids. 

 

Sample Requirements

aSample concentrations must be measured by fluorometric method, such as Qubit or PicoGreen, otherwise they will not be processed.
bAllows for sizing of high molecular weight DNA between 75 - 48,500 bp.
Sample Type Concentrationa (ng/µl) Minimum volume (µl) Number of Samples/Run Cost/Run
Total RNA 1 - 5 10 11 $56 for the first run, each additional run is $50
Submitter Prepared Libraries or dsDNA PCR products

0.5 - 5

5

11

$56 for the first run, each additional run is $50
Genomic DNAb 25 - 250 5 11 $56 for the first run, each additional run is $50

Submitting Samples for Analysis:

For 1-22 samples, samples can be submitted in PCR strip-tubes.  For >22 samples, samples must be submitted in a 96-well PCR-type plate, filled by row, column 12 MUST be left empty.  All tubes and plates must be clearly labeled.  If you cannot clearly label the tubes or plate, you must place them in a secondary container that is properly labeled.

Samples must be submitted to the Genomics Core ready to be processed on either the Bioanalyzer or the Fragment Analyzer.  Samples must be at the appropriate concentration in the correct volume, specified in table above.  The Genomics Core will not quantify or dilute samples submitted for analysis.  Samples that are not submitted as specified will not be processed.  Samples submitted for analysis will not be returned to researcher.

Researchers will pre-register their samples in the LIMS and deliver their samples to the Genomics Core along with the LIMS submission sheet.  Place samples in the -20 freezer (bin labeled "Bioanalyzer/Fragment Analyzer") in the hallway outside of the Genomics Core (S-18 Plant Biology).  Place the LIMS submission form in the basket labeled "Bioanalyzer/Fragment Analyzer" that is attached to the outside of the freezer.  For samples delivered by 10 AM, results will be available on the Genomics Depot within 48 hours.

There are two submission spreadsheets available for submitting your samples.  If submitting 1-22 samples in PCR strip-tubes, use form DNA-RNA_SubmitForQC_Tubes.xlsx.  If you are submitting samples in a 96-well plate, use form DNA-RNA_SubmitForQC_96WellPlate.xlsx.