Bioanalyzer/TapeStation Analysis

 

The Genomics Core has many Agilent 2100 Bioanalyzer and 4200 TapeStation kits available for sample analysis of total RNA, submitter prepared libraries, dsDNA PCR products, fragmented DNA, and high molecular weight genomic DNA. Genomics Core Staff will select the appropriate kit based on details provided by the researcher at time of submission. 

The table below describes the specifications for submitting samples to the Genomics Core for analysis on the Agilent 2100 Bioanalyzer or the 4200 TapeStation.  Both instruments use automated electrophoresis for sizing nucleic acids. 

 

Sample Requirements

aSample concentrations must be measured by fluorometric method, such as Qubit or PicoGreen, otherwise they will not be processed.

bAllows for sizing of high molecular weight DNA between 200 - 60,000 bp.

Sample Type Sizing Range (bp) Concentrationa (ng/µl) Minimum volume (µl) Number of Samples/Run Cost/Run
Total RNA not applicable 1 - 5 10 11 $56 for the first run, each additional run is $50
Prepared libraries, PCR products, or fragmented DNA Bioanalyzer: 50 - 7,000 bp Tapestation: 35 - 1,000 bp 0.5 - 1 5

11

$56 for the first run, each additional run is $50
Genomic DNAb 200 - 60,000 bp 25 - 50 5 11 $56 for the first run, each additional run is $50

Submitting Samples for Analysis:

For 15 samples or less, samples can be submitted in PCR strip-tubes or 1.5 ml microcentrifuge tubes.  For 16 or more samples, samples must be submitted in a 96-well PCR-type plate, filled by column.  All tubes and plates must be clearly labeled.  If you cannot clearly label the tubes or plate, you must place them in a secondary container that is properly labeled.

Samples must be submitted to the Genomics Core ready to be processed on either the Bioanalyzer or TapeStation.  Samples must be at the appropriate concentration in the correct volume, specified in table above.  The Genomics Core will not quantify or dilute samples submitted for analysis.  Samples that are not submitted as specified will not be processed.  Samples submitted for analysis will not be returned to researcher and will be discarded after 2 weeks.

Researchers will pre-register their samples in the LIMS and deliver their samples to the Genomics Core along with the LIMS submission sheet.  Place samples in the -20 freezer (bin labeled "Bioanalyzer/TapeStation") in the hallway outside of the Genomics Core (S-18 Plant Biology).  Place the LIMS submission form in the basket labeled "Bioanalyzer/TapeStation" that is attached to the outside of the freezer.  Results are typically available on Genomics Depot within 48 business hours.

If you do not have a LabLink account, you will need to request one in order to submit samples for this service.  See our ClarityLIMS LabLink page for information about requesting an account, creating a project, and submission forms.