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Sanger Sequencing Standard PCR Cycling Conditions

The default PCR cycling conditions for Standard Reactions and 1st Tier Reactions:

94˚C for 5:00
45 cycles:
 95˚C for 0:10  denaturing
 50˚C for 0:05  annealing
 60˚C for 2:30  extension
Hold 4˚C