Sanger Sequencing Standard PCR Cycling Conditions
The default PCR cycling conditions for Standard Reactions and 1st Tier Reactions:
94˚C for 5:00
45 cycles:
95˚C for 0:10 denaturing
50˚C for 0:05 annealing
60˚C for 2:30 extension
Hold 4˚C
The RTSF Mass Spectrometry and Metabolomics Core has temporarily moved to room 116 on the first floor in the Biochemistry building to allow for renovation of our basement facility space. Core operations will continue as normal in room 116 until the basement renovations are completed.
More InformationThe default PCR cycling conditions for Standard Reactions and 1st Tier Reactions:
94˚C for 5:00
45 cycles:
95˚C for 0:10 denaturing
50˚C for 0:05 annealing
60˚C for 2:30 extension
Hold 4˚C