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Rates

MSU TGEF Current Recharge Rates

Listed rates are for complete projects only, except where individual services are listed. Complete projects include all steps from design to cloning of targeting vector, to validation of the final genome edited founder or clonal cell line. In addition, we accommodate projects where constructs or molecular reagents are provided by the investigator, at lower rates. Please contact our staff directly if you need a breakdown of the rates or more details for a proposed budget. All payment methods, except for MSU accounts, will be subject to a 2% University Administrative Fee.


Note: Listed rates are for internal MSU users, rates for External User projects will be marked up 80% over cost information listed.

 

I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection

II. Cryopreservation

III. Embryo Transfer Surgery, IVF and Rederivation Services

IV. Individual Services, Equipment Use, Animal Per Diems, Labor Charges

V. Genome Edited Cell Line Models

 

I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection

Base internal rates are listed for commonly used genomic background strains - C57BL/6, FVB/N, CD1 for mice, and SAS (Sprague Dawley) for rats. Additional charges may apply if different strains than those listed are used. Rates are calculated for a fixed number of embryos or founders per project, if that number is exceeded additional charges may apply. A detailed modular billing structure can be downloaded as a PDF here.

Service ID Description Total Cost MOUSE Total Cost RAT
C1KO-M/R CRISPR KO base rate for B6 background $3,115.64 $4008.79
C2SM-M/R CRISPR SM <120bp, SNP, Deletions $3,416.68 $4,831.14
C3CK1-M/R CRISPR Conditional KO (LoxPs <3kb apart) $9,609.91 $11,397.94
C3CK2-M/R CRISPR Conditional KO (LoxPs >3kb apart) $10,677.25 $12,465.28
C4KI1-M/R CRISPR KI (large insertion) 120bp - 1kb $6,854.65 $8,195.24
C4KI2-M/R CRISPR KI (large insertion) 1kb - 3kb $7,482.60 $8,823.19
C4KI3-M/R CRISPR KI (large insertion) >3kb $9,637.34 $10,977.93
C5SFTG-M/R CRISPR Safe Harbor Transgene Knockin $12,563.56 $14,351.59
TG-M/R Conventional Transgenic $10,087.77 $11,427.50
ESM-M/R Microinjection of ES cells (includes clone expansion and microinjection of up to 3 clones) $5,547.99 $6,782.53

II. Cryopreservation

Model Description Service ID Total Cost Extra Session Extra charge if > 5 females
Mouse Sperm Cryopreservation (per session) SCP-M $297.05    
Mouse Embryo Cryopreservation (per session; 1-5 females) ECP-M $297.39 $212.13 $86.52
Rat Embryo Cryopreservation (per session; 1-5 females) ECP-R $297.39 $212.13 $86.52

III. Embryo Transfer Surgery, IVF and Rederivation Services

Model or Category Description Service ID Total Cost
Mouse Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion ETS-M $751.99
Rat Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion ETS-R $735.92
Mouse Mouse IVF (not including embryo transfer), required for speed congenics or expansion, cryorecovery from sperm IVF-M $534.40
Mouse Mouse Rederivation through IVF base rate MsRed-IVF $1,286.39

IV. Individual Services, Equipment Use, Animal Per Diems, Labor Charges

A la carte billing is offered for individual services, equipment use, or indidivual animal or reagents costs to provide options for custom projects.

Category Service Description Service ID Total Cost
Equipment Neon Nucleofector initial training and set up NN-T $135.84
Equipment Neon Nucleofector consumables per rxn NN-C $11.88
Equipment Tissue Culture Suite Use per hr TCS-HR $25.00
Equipment Liquid Nitrogen storage per box/project CPB $28.42
Equipment Liquid Nitrogen Dry Shipper Rental (per shipment). Hourly rate will be charged if the shipper needs to be filled DS $59.05
Molecular Services Custom Construct Cloning MOL $2,394.20
Sequencing Amplicon NGS (per reaction) NGS-SEQ $50.00
Sequencing Sanger Sequencing (per reaction) SEQ $5.00
Molecular Services Plasmid expansion and purification PLAS $290.00
Synthetic NN Nucleic acid synthesis (cost per bp) SynNN $0.25
Synthetic NN DNA oligo ave cost Oligo $3.30
Synthetic NN Single guide RNA (gRNA) synthetic, modified gRNA $99.00
Synthetic NN Ultramer (ssODN) ave cost ssODN $110.21
Molecular Services Genotyping base rate (per round) for established lines with defined protocols GT-BR $114.33
Molecular Services Genotyping per reaction, in addition to base rate per round GT-Rxn $6.25
Molecular Services Geno Strategy Development per locus GSD $339.29
Molecular Services CRISPR Off-target analysis up to 3 hits, based on PCR amplification and Sanger sequencing of off-target hit loci OTA $392.69
Labor Hourly Rate for TGEF personnel labor including consultation, training and editing LH $79.33
Per diems Animal housing per cage per day for Mice PD-M $1.19
Per diems Animal housing per cage per day for Rats PD-R $1.36
Animal purchase Non-standard strain animal costs / unit STR $10.00
Shipping Shipping charges /unit SHIP $10.00
Ear tagging Ear tagging and tail cutting per animal TAG $5.48

 

Category Service Description Service ID Total Cost Extra charge if >100 embryos, cost/25 embryos (pronuclear or 2-cell stage) or cost/20 embryos (8-cell to blastocyst stage)
Embryo CD1 mouse electroporation, 100 embryos CD1e- $1,537.53 $384.38
Embryo CD1 mouse pronuclear microinjection, 100 embryos CD1-PNMJ $2,149.97 $537.49
Embryo CD1 2C microinjection, 100 embryos CD1-2CMJ $2,308.63 $577.16
Embryo CD1 ESC microinjection, 100 embryos CD1-ESMJ $2,351.05 $470.21
Embryo B6 mouse electroporation, 100 embryos B6e- $1,713.38 $428.35
Embryo B6 mouse pronuclear microinjection, 100 em B6-PNMJ $2,325.82 $581.45
Embryo B6 mouse 2C microinjection, 100 embryos B6-2CMJ $2,524.15 $631.04
Embryo Mouse ESC microinjection, 100 embryos B6-ESMJ $2,751.68 $550.34
Embryo SD rat electroporation, 100 embryos SDe- $2,606.54 $651.63
Embryo SD rat pronuclear microinjection, 100 embryos SD-PNMJ $3,218.97 $804.74
Embryo Rat 2C microinjection, 100 embryos PNMJ-R $3,417.30 $854.33
Embryo Rat ESC microinjection, 100 embryos ESMJ-R $3,574.70 $714.94

V. Genome Edited Cell Line Models

Model or Category Project Type Service ID Total Cost
Individual Services Tissue culture base fee for cell line derivation, construct validation, transfection, or other tests (additional labor costs may apply). TC $870.94
Cell Line CRISPR KO - targeted heterologous pool C6CLKO Pool custom project budget
Cell Line CRISPR KO - clonal line expansion and screening C6CLKO Clone custom project budget
Cell Line CRISPR Small modification ( <120bp insertion, SNP, deletion) with clonal expansion and screening C7CLSM Clone custom project budget
Cell Line CRISPR Large insertion/knockin (>120bp) C7CLKI Clone custom project budget
Cell Line Transgenic or safe harbor locus targeting C8TG custom project budget
Mouse ESCs ESC targeting EST custom project budget