Rates
MSU TGEF Current Recharge Rates
Listed rates are for complete projects only, except where individual services are listed. Complete projects include all steps from design to cloning of targeting vector, to validation of the final genome edited founder or clonal cell line. In addition, we accommodate projects where constructs or molecular reagents are provided by the investigator, at lower rates. Please contact our staff directly if you need a breakdown of the rates or more details for a proposed budget. All payment methods, except for MSU accounts, will be subject to a 2% University Administrative Fee.
Note: Listed rates are for internal MSU users, rates for External User projects will be marked up 80% over cost information listed.
III. Embryo Transfer Surgery, IVF and Rederivation Services
IV. Individual Services, Equipment Use, Animal Per Diems, Labor Charges
V. Genome Edited Cell Line Models
I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection
Base internal rates are listed for commonly used genomic background strains - C57BL/6, FVB/N, CD1 for mice, and SAS (Sprague Dawley) for rats. Additional charges may apply if different strains than those listed are used. Rates are calculated for a fixed number of embryos or founders per project, if that number is exceeded additional charges may apply. A detailed modular billing structure can be downloaded as a PDF here.
| Service ID | Description | Total Cost MOUSE | Total Cost RAT |
|---|---|---|---|
| C1KO-M/R | CRISPR KO base rate for B6 background | $3,115.64 | $4008.79 |
| C2SM-M/R | CRISPR SM <120bp, SNP, Deletions | $3,416.68 | $4,831.14 |
| C3CK1-M/R | CRISPR Conditional KO (LoxPs <3kb apart) | $9,609.91 | $11,397.94 |
| C3CK2-M/R | CRISPR Conditional KO (LoxPs >3kb apart) | $10,677.25 | $12,465.28 |
| C4KI1-M/R | CRISPR KI (large insertion) 120bp - 1kb | $6,854.65 | $8,195.24 |
| C4KI2-M/R | CRISPR KI (large insertion) 1kb - 3kb | $7,482.60 | $8,823.19 |
| C4KI3-M/R | CRISPR KI (large insertion) >3kb | $9,637.34 | $10,977.93 |
| C5SFTG-M/R | CRISPR Safe Harbor Transgene Knockin | $12,563.56 | $14,351.59 |
| TG-M/R | Conventional Transgenic | $10,087.77 | $11,427.50 |
| ESM-M/R | Microinjection of ES cells (includes clone expansion and microinjection of up to 3 clones) | $5,547.99 | $6,782.53 |
II. Cryopreservation
| Model | Description | Service ID | Total Cost | Extra Session | Extra charge if > 5 females |
|---|---|---|---|---|---|
| Mouse | Sperm Cryopreservation (per session) | SCP-M | $297.05 | ||
| Mouse | Embryo Cryopreservation (per session; 1-5 females) | ECP-M | $297.39 | $212.13 | $86.52 |
| Rat | Embryo Cryopreservation (per session; 1-5 females) | ECP-R | $297.39 | $212.13 | $86.52 |
III. Embryo Transfer Surgery, IVF and Rederivation Services
| Model or Category | Description | Service ID | Total Cost |
|---|---|---|---|
| Mouse | Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion | ETS-M | $751.99 |
| Rat | Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion | ETS-R | $735.92 |
| Mouse | Mouse IVF (not including embryo transfer), required for speed congenics or expansion, cryorecovery from sperm | IVF-M | $534.40 |
| Mouse | Mouse Rederivation through IVF base rate | MsRed-IVF | $1,286.39 |
IV. Individual Services, Equipment Use, Animal Per Diems, Labor Charges
A la carte billing is offered for individual services, equipment use, or indidivual animal or reagents costs to provide options for custom projects.
| Category | Service Description | Service ID | Total Cost |
|---|---|---|---|
| Equipment | Neon Nucleofector initial training and set up | NN-T | $135.84 |
| Equipment | Neon Nucleofector consumables per rxn | NN-C | $11.88 |
| Equipment | Tissue Culture Suite Use per hr | TCS-HR | $25.00 |
| Equipment | Liquid Nitrogen storage per box/project | CPB | $28.42 |
| Equipment | Liquid Nitrogen Dry Shipper Rental (per shipment). Hourly rate will be charged if the shipper needs to be filled | DS | $59.05 |
| Molecular Services | Custom Construct Cloning | MOL | $2,394.20 |
| Sequencing | Amplicon NGS (per reaction) | NGS-SEQ | $50.00 |
| Sequencing | Sanger Sequencing (per reaction) | SEQ | $5.00 |
| Molecular Services | Plasmid expansion and purification | PLAS | $290.00 |
| Synthetic NN | Nucleic acid synthesis (cost per bp) | SynNN | $0.25 |
| Synthetic NN | DNA oligo ave cost | Oligo | $3.30 |
| Synthetic NN | Single guide RNA (gRNA) synthetic, modified | gRNA | $99.00 |
| Synthetic NN | Ultramer (ssODN) ave cost | ssODN | $110.21 |
| Molecular Services | Genotyping base rate (per round) for established lines with defined protocols | GT-BR | $114.33 |
| Molecular Services | Genotyping per reaction, in addition to base rate per round | GT-Rxn | $6.25 |
| Molecular Services | Geno Strategy Development per locus | GSD | $339.29 |
| Molecular Services | CRISPR Off-target analysis up to 3 hits, based on PCR amplification and Sanger sequencing of off-target hit loci | OTA | $392.69 |
| Labor | Hourly Rate for TGEF personnel labor including consultation, training and editing | LH | $79.33 |
| Per diems | Animal housing per cage per day for Mice | PD-M | $1.19 |
| Per diems | Animal housing per cage per day for Rats | PD-R | $1.36 |
| Animal purchase | Non-standard strain animal costs / unit | STR | $10.00 |
| Shipping | Shipping charges /unit | SHIP | $10.00 |
| Ear tagging | Ear tagging and tail cutting per animal | TAG | $5.48 |
| Category | Service Description | Service ID | Total Cost | Extra charge if >100 embryos, cost/25 embryos (pronuclear or 2-cell stage) or cost/20 embryos (8-cell to blastocyst stage) |
|---|---|---|---|---|
| Embryo | CD1 mouse electroporation, 100 embryos | CD1e- | $1,537.53 | $384.38 |
| Embryo | CD1 mouse pronuclear microinjection, 100 embryos | CD1-PNMJ | $2,149.97 | $537.49 |
| Embryo | CD1 2C microinjection, 100 embryos | CD1-2CMJ | $2,308.63 | $577.16 |
| Embryo | CD1 ESC microinjection, 100 embryos | CD1-ESMJ | $2,351.05 | $470.21 |
| Embryo | B6 mouse electroporation, 100 embryos | B6e- | $1,713.38 | $428.35 |
| Embryo | B6 mouse pronuclear microinjection, 100 em | B6-PNMJ | $2,325.82 | $581.45 |
| Embryo | B6 mouse 2C microinjection, 100 embryos | B6-2CMJ | $2,524.15 | $631.04 |
| Embryo | Mouse ESC microinjection, 100 embryos | B6-ESMJ | $2,751.68 | $550.34 |
| Embryo | SD rat electroporation, 100 embryos | SDe- | $2,606.54 | $651.63 |
| Embryo | SD rat pronuclear microinjection, 100 embryos | SD-PNMJ | $3,218.97 | $804.74 |
| Embryo | Rat 2C microinjection, 100 embryos | PNMJ-R | $3,417.30 | $854.33 |
| Embryo | Rat ESC microinjection, 100 embryos | ESMJ-R | $3,574.70 | $714.94 |
V. Genome Edited Cell Line Models
| Model or Category | Project Type | Service ID | Total Cost |
|---|---|---|---|
| Individual Services | Tissue culture base fee for cell line derivation, construct validation, transfection, or other tests (additional labor costs may apply). | TC | $870.94 |
| Cell Line | CRISPR KO - targeted heterologous pool | C6CLKO Pool | custom project budget |
| Cell Line | CRISPR KO - clonal line expansion and screening | C6CLKO Clone | custom project budget |
| Cell Line | CRISPR Small modification ( <120bp insertion, SNP, deletion) with clonal expansion and screening | C7CLSM Clone | custom project budget |
| Cell Line | CRISPR Large insertion/knockin (>120bp) | C7CLKI Clone | custom project budget |
| Cell Line | Transgenic or safe harbor locus targeting | C8TG | custom project budget |
| Mouse ESCs | ESC targeting | EST | custom project budget |