Grant Support

The Proteomics Core has at its disposal two state-of-the-art LC/MS/MS mass spectrometers; a Thermo Scientific Q-Exactive interfaced with a Thermo Scientific EASY nLC 1200 UPLC and a Thermo Scientific Excedion Pro with extended mass range and EASY EThcD capabilities interfaced with a Thermo Scientific Vanquish NEO UPLC, both with Flex-Spray Ionization Sources.  These systems combine unique High Resolution/Accurate Mass measurement and high sensitivity Orbitrap detection with exceptional mass selection and spectral multiplexing over extended, stable LC gradients making them ideal for proteome profiling of complex samples and highly specific peptide quantitation.  Deep proteome mapping can be achieved using a Waters Acquity H-class UPLC for offline MUDPIT-style analyses.  Software assets include Mascot Distiller, Proteome Discoverer, MaxQuant and Skyline and Scaffold software suites.  Mascot, SEQUEST, MSFragger and Andromeda search algorithms are available for peptide-to-spectrum matching and quantitation.