Rates
MSU TGEF Current Recharge Rates
Listed rates are for complete projects only, except where individual services are listed. Complete projects include all steps from design to cloning of targeting vector, to validation of the final genome edited founder or clonal cell line. In addition, we accommodate projects where constructs or molecular reagents are provided by the investigator, at lower rates. Please contact our staff directly if you need a breakdown of the rates or more details for a proposed budget. All payment methods, except for MSU accounts, will be subject to a 2% University Administrative Fee.
Note: Listed rates are for internal MSU users, rates for External User projects will
be marked up 26% over cost information listed.
III. Cryopreservation and cryostorage
IV. Embryo Transfer, IVF and Rederivation Services
V. Genome Edited Cell Line Models
VI. Genome Editing or Epigenetic regulation in Cell Lines
I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection
Cost for animal models includes the generation of confirmed F1 transmission animals from selected edited founders. Base rates are listed for commonly used genomic background strains - C57BL6 for mice, and SAS (Sprague Dawley) for rats. Additional charges may apply if different strains than those listed are used. Rates are calculated for a fixed number of embryos, founders and F1 offspring per project, if that number is exceeded additional charges will apply. A detailed modular billing structure can be downloaded as a PDF Here
| Service ID | Description | Total Cost MOUSE | Total Cost RAT |
|---|---|---|---|
| C1KO-M/R | CRISPR KO | $4,872.94 | $6,340.52 |
| C2SM-M/R | CRISPR SM <120bp, SNP, Deletions | $5,242.11 | $7,334.09 |
| C3CK-M/R | CRISPR Conditional KO | $9,654.11 | $11,706.55 |
| C4KI1-M/R | CRISPR KI (large insertion) 120bp - 1kb | $8,315.78 | $10,592.70 |
| C4KI2-M/R | CRISPR KI (large insertion) 1kb - 3kb | $9,394.40 | $11,307.80 |
| C4KI3-M/R | CRISPR KI (large insertion) >3kb | $14,007.14 | $14,594.25 |
| TG-M/R | Conventional Transgenic | $10,722.31 | $12,497.45 |
| ESM-M/R | Microinjection of ES cells (includes clone expansion and microinjection of up to 3 clones) | $6,227.38 | $7,953.93 |
For users interested only in embryo manipulation services, we provide microinjection and electroporation of user-provide DNA/RNA/protein
| Service ID | Service Description | Total Cost | Extra chrg if >100 emb, cost/25 emb (PN & 2C) or cost/20 emb (8-cell - blast) |
| CD1e- | CD1 mouse electroporation, 100 embryos embryos per session | $1790.65 | $447.66 |
| CD1-PNMJ | CD1 mouse pronuclear microinjection, 100 embryos per session | $2021.98 | $505.49 |
| CD1-2CMJ | CD1 2C microinjection, 100 embryos | $2203.88 | $550.97 |
| CD1-ESMJ | CD1 ESC microinjection, 100 embryos | $2272.88 | $454.58 |
| B6e- | B6 mouse electroporation, 100 embryos | $1943.06 | $485.76 |
| B6-PNMJ | B6 mouse pronuclear microinjection, 100 em | $2310.81 | $577.70 |
| B6-2CMJ | B6 mouse 2C microinjection, 100 embryos | $2401.77 | $600.44 |
| B6-ESMJ | Mouse ESC microinjection, 100 embryos | $2734.96 | $546.99 |
| SDe- | SD rat electroporation, 100 embryos | $3121.97 | $780.49 |
| SDmj | SD rat microinjection, 100 embryos | $3494.24 | $873.56 |
| PNMJ-R | Rat 2C microinjection, 100 embryos | $3585.19 | $896.30 |
| ESMJ-R | Rat ESC microinjection, 100 embryos | $3886.00 | $777.20 |
II. Additional F1 charges for Genome Edited models above base rate can be found Here
III. Cryopreservation and cryostorage
| Model | Description | Service ID | Total Cost | Extra Session | Extra charge if > 5 females |
|---|---|---|---|---|---|
| Mouse | Sperm Cryopreservation (per session) | SCP-M | $368.01 | ||
| Mouse | Embryo Cryopreservation (per session; 1-5 females) | ECP-M | $347.08 | $227.60 | $83.59 |
| Rat | Embryo Cryopreservation (per session; 1-5 females) | ECP-R | $347.08 | $227.60 | $83.59 |
| Category | Service Description | Service ID | Total Cost |
| Equipment | Liquid Nitrogen storage per box/project yearly | CPB | $36.32 |
| Equipment | Liquid Nitrogen Dry Shipper Rental (per shipment). Hourly rate charged if the shipper needs to be filled | DS | $59.05 |
IV. Embryo Transfer, IVF and Rederivation Services
| Model or Category | Description | Service ID | Total Cost |
|---|---|---|---|
| Mouse | Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion | ETS-M | $890.98 |
| Rat | Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion | ETS-R | $1006.83 |
| Mouse | Mouse IVF (not including embryo transfer), required for speed congenics or expansion, cryorecovery from sperm | IVF-M | $811.67 |
| Mouse | Mouse Rederivation through IVF base rate | MsRed-IVF | $1,702.65 |
V. Individual Services, Equipment Use, Animal Per Diems, Labor Charges
A la carte billing is offered for individual services, equipment use, or individual animal or reagents costs to provide options for custom projects. A full list of these rates can be found Here
| Category | Service Description | Service ID | Total Cost |
|---|---|---|---|
| Labor | Hourly TGEF RAI personnel rate | LH-RAI | $43.73 |
| Labor | Hourly TGEF RS-MB personnel rate | LH-MB | $75.35 |
| Labor | Hourly TGEF RS-RB personnel rate | LH-RB | $90.95 |
| Equipment | Fragment Analyzer per round (up to 11) | FA-R | $15.11 |
| Equipment | Fragment Analyzer consumables per rxn | FA-C | $1.01 |
| Molecular Services | Genotyping base rate (per round) | GT-BR | $235.64 |
| Molecular Services | Genotyping base rate (per rxn) | GT-Rxn | $6.55 |
| Molecular Services | Genotyping per sample T7 assay or RE digest | GT-D | $4.15 |
| Molecular Services | Genotyping with additional Sanger seq per sample | GT-S | $8.38 |
| Molecular Services | Geno Strategy Development per locus | GSD | $398.54 |
| Molecular Services | CRISPR Off-target analysis up to 3 hits | OTA | $415.30 |
| Per diems | Animal housing per cage per day for Mice | PD-M | $1.39 |
| Per diems | Animal housing per cage per day for Rats | PD-R | $1.44 |
VI. Genome Editing or Epigenetic regulation in Cell Lines
We provide consultation or in-house services for Genome editing or CRISPR epigenetic regulation for cell lines based on custom project rates.