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Rates

MSU TGEF Current Recharge Rates

Listed rates are for complete projects only, except where individual services are listed. Complete projects include all steps from design to cloning of targeting vector, to validation of the final genome edited founder or clonal cell line. In addition, we accommodate projects where constructs or molecular reagents are provided by the investigator, at lower rates. Please contact our staff directly if you need a breakdown of the rates or more details for a proposed budget. All payment methods, except for MSU accounts, will be subject to a 2% University Administrative Fee.


Note: Listed rates are for internal MSU users, rates for External User projects will be marked up 26% over cost information listed.

 

I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection

II. Additional F1 charges

III. Cryopreservation and cryostorage

IV. Embryo Transfer, IVF and Rederivation Services

V. Genome Edited Cell Line Models

VI. Genome Editing or Epigenetic regulation in Cell Lines


I. Genome Edited Animal Models: Mouse, Rat, CRISPR-based, Conventional Transgenics, ES cell microinjection

Cost for animal models includes the generation of confirmed F1 transmission animals from selected edited founders. Base rates are listed for commonly used genomic background strains - C57BL6 for mice, and SAS (Sprague Dawley) for rats.  Additional charges may apply if different strains than those listed are used.  Rates are calculated for a fixed number of embryos, founders and F1 offspring per project, if that number is exceeded additional charges will apply.  A detailed modular billing structure can be downloaded as a PDF Here

Service ID Description Total Cost MOUSE Total Cost RAT
C1KO-M/R CRISPR KO  $4,872.94 $6,340.52
C2SM-M/R CRISPR SM <120bp, SNP, Deletions $5,242.11 $7,334.09
C3CK-M/R CRISPR Conditional KO $9,654.11 $11,706.55
C4KI1-M/R CRISPR KI (large insertion) 120bp - 1kb $8,315.78 $10,592.70
C4KI2-M/R CRISPR KI (large insertion) 1kb - 3kb $9,394.40 $11,307.80
C4KI3-M/R CRISPR KI (large insertion) >3kb $14,007.14 $14,594.25
TG-M/R Conventional Transgenic $10,722.31 $12,497.45
ESM-M/R Microinjection of ES cells (includes clone expansion and microinjection of up to 3 clones) $6,227.38 $7,953.93

For users interested only in embryo manipulation services, we provide microinjection and electroporation of user-provide DNA/RNA/protein

Service ID Service Description Total Cost Extra chrg if >100 emb, cost/25 emb (PN & 2C) or cost/20 emb (8-cell - blast)
CD1e- CD1 mouse electroporation, 100 embryos embryos per session $1790.65 $447.66
CD1-PNMJ CD1 mouse pronuclear microinjection,  100 embryos per session $2021.98 $505.49
CD1-2CMJ CD1 2C microinjection, 100 embryos $2203.88 $550.97
CD1-ESMJ CD1 ESC microinjection, 100 embryos $2272.88 $454.58
B6e- B6 mouse electroporation, 100 embryos $1943.06 $485.76
B6-PNMJ B6 mouse pronuclear microinjection, 100 em $2310.81 $577.70
B6-2CMJ B6 mouse 2C microinjection, 100 embryos $2401.77 $600.44
B6-ESMJ Mouse ESC microinjection, 100 embryos $2734.96 $546.99
SDe- SD rat electroporation, 100 embryos $3121.97 $780.49
SDmj SD rat microinjection, 100 embryos $3494.24 $873.56
PNMJ-R Rat 2C microinjection,  100 embryos $3585.19 $896.30
ESMJ-R Rat ESC microinjection, 100 embryos $3886.00 $777.20

 

II. Additional F1 charges for Genome Edited models above base rate can be found Here

III. Cryopreservation and cryostorage

Model Description Service ID Total Cost Extra Session Extra charge if > 5 females
Mouse Sperm Cryopreservation (per session) SCP-M $368.01    
Mouse Embryo Cryopreservation (per session; 1-5 females) ECP-M $347.08 $227.60 $83.59
Rat Embryo Cryopreservation (per session; 1-5 females) ECP-R $347.08 $227.60 $83.59

 

Category Service Description Service ID Total Cost
Equipment Liquid Nitrogen storage per box/project yearly CPB $36.32
Equipment Liquid Nitrogen Dry Shipper Rental (per shipment). Hourly rate charged if the shipper needs to be filled DS $59.05

 

IV. Embryo Transfer, IVF and Rederivation Services

Model or Category Description Service ID Total Cost
Mouse Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion ETS-M $890.98
Rat Embryo transfer surgery: required for rederivation, cryorecovery, speed congenics and speed expansion ETS-R $1006.83
Mouse Mouse IVF (not including embryo transfer), required for speed congenics or expansion, cryorecovery from sperm IVF-M $811.67
Mouse Mouse Rederivation through IVF base rate MsRed-IVF $1,702.65

V. Individual Services, Equipment Use, Animal Per Diems, Labor Charges

A la carte billing is offered for individual services, equipment use, or individual animal or reagents costs to provide options for custom projects. A full list of these rates can be found Here

Category Service Description Service ID Total Cost
Labor Hourly TGEF RAI personnel rate  LH-RAI $43.73
Labor Hourly TGEF RS-MB personnel rate   LH-MB $75.35
Labor Hourly TGEF RS-RB personnel rate LH-RB $90.95
Equipment Fragment Analyzer per round (up to 11) FA-R $15.11
Equipment Fragment Analyzer consumables per rxn FA-C $1.01
Molecular Services Genotyping base rate (per round) GT-BR $235.64
Molecular Services Genotyping base rate (per rxn) GT-Rxn $6.55
Molecular Services Genotyping per sample T7 assay or RE digest  GT-D $4.15
Molecular Services Genotyping with additional Sanger seq per sample GT-S $8.38
Molecular Services Geno Strategy Development per locus GSD $398.54
Molecular Services CRISPR Off-target analysis up to 3 hits OTA $415.30
Per diems Animal housing per cage per day for Mice PD-M $1.39
Per diems Animal housing per cage per day for Rats PD-R $1.44

VI. Genome Editing or Epigenetic regulation in Cell Lines


We provide consultation or in-house services for Genome editing or CRISPR epigenetic regulation for cell lines based on custom project rates.