Genotyping
The RTSF Genomics Core at Michigan State University offers a number of options for genotyping services. The available platforms and technologies encompass a wide range of sample and assay set sizes allowing analysis of a handful to millions of loci simultaneously.
GeneScan/Fragment Analysis Services
Fragment Analysis Service to End
We will end fragment analysis services that run on the ABI 3730XL. There are not
enough fragment analysis projects to justify keeping the 3730XL. For fragment analysis,
services will end when we run out of size standards. Size standards may last longer
than Sanger BigDye, but in any case, we will cease all services on the 3730XL in September
when the service contract for the instrument expires. Please contact us before submitting
any fragment analysis samples.
Amplified Fragment Length Polymorphism (AFLP)
AFLP is a powerful DNA fingerprinting technique that can be used for plants and bacteria based on the selective amplification of restriction fragments from a total digest of genomic DNA.
Short Tandem Repeats (STR)
STR analysis is an important complement to both the length and sequence-based DNA typing systems used for human identification. Due to their small size STRs are often used successfully to analyze samples containing minute amounts and degraded DNA that cannot be typed using conventional methods. STRs are commonly used for forensic applications determining population genotype frequencies.
Recommended DNA quantity for GeneScan/Fragment Analysis: 200-300 ng of DNA in a 20 µL volume. DNA must be quantified by fluorometric method, such as Qubit or PicoGreen.
Please contact the Genomics Core (gtsf@msu.edu) for more information.