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Pricing

Pricing is identical for MSU researchers and for off-campus researchers.  However, payments from non-MSU accounts (i.e. off-campus researchers) will be subject to a 3% University Administrative Fee.  MSU also requires the addition of a 26% Facilities and Administrative Cost charge to services provided to for-profit companies.


Sample Quality Control Services

The MSU Genomics Core offers Biotium Quantification Kits for use with the Qubit.  For accurate quantification, the two kit standards must be measured with each batch of samples that are processed.  The standards are charged at the same rate as a sample, therefore, if you are quantifying two samples the cost will be $4 (4 x $1.00) because of the two required standards.

DESCRIPTION SAMPLE TYPE AND RANGE PRICE

AccuGreen Broad Range
dsDNA Quantification, each

Double-stranded DNA
2 - 1000 ng

$1.00
AccuGreen High Sensitivity
dsDNA Quantification, each
Double-stranded DNA
0.1 - 100 ng
$1.00
AccuBlue Broad Range
RNA Quantification, each
Total RNA
5 - 1000 ng
$1.00

Visit the Common Use Equipment webpage for instructions to reserve the qubit.

A minimum of 72 samples are required for this service.

Qubit Flex Quantification Sample Requirements
Qubit Flex Quantification Submission Form

KIT

SAMPLE TYPE
AND RANGE
PRICE FOR FIRST 72 SAMPLES PRICE FOR EACH ADDITIONAL COLUMN*
AccuGreen Broad Range
dsDNA Quantification
Double‑stranded DNA
2 - 1000 ng
$163 $19
AccuGreen High Sensitivity
dsDNA Quantification
Double‑stranded DNA
0.1 - 100 ng
$176 $20
AccuBlue Broad Range
RNA Quantification
Total RNA
5 - 1000 ng
$156 $18

*Each column can have up to 8 samples.  Partially filled columns will be charged at the column price.

The TapeStation is an automated electrophoresis instrument that can assess the quality and/or size of nucleic acids.  It performs the same function as a bioanalyzer, but allows for high-throughput sample processing.

TapeStation Sample Requirements and Service Description
TapeStation Submission Form

DESCRIPTION QUANTITY PRICE PER SAMPLE

HS RNA Assay

For quality assessment
of total RNA.

≤ 6 samples $14 (minimum fee $36)
7 - 47 samples $10
≥ 48 samples $7

D1000 HS assay

For sizing and quality assessment of double‑stranded DNA up to 1 kb. 

Examples include prepared libraries, fragmented DNA, and PCR products.

≤ 6 samples $13 (minimum fee $28)
7 - 47 samples $10
≥ 48 samples $8

D5000 HS assay

For sizing and quality assessment of double‑stranded DNA up to 5 kb. 

Examples include cDNA, ATAC-seq libraries and other large insert libraries.

≤ 6 samples $17 (minimum fee $28)
7 - 47 samples $10
≥ 48 samples $8

Genomic DNA assay

For sizing of high molecular weight (HMW) Genomic DNA.

≤ 6 samples $18 (minimum fee $29)
7 - 47 samples $11
≥ 48 samples $9

 

AVITI/Illumina (Short-Read) Library Preparation & Sequencing

Genomic DNA Library Preparation Services

The Genomics Core is now offering Bundled Flat Rate Pricing for DNA library preparation and sequencing on the AVITI.  This service allows researchers to pay a flat rate for library preparation and a set amount of sequencing for each sample.  The Quantabio SparQ DNA library prep method will be used for this service.

DNA sample requirements are given on our Sample Requirements webpage.

The Bundled Flat Rate Submission Forms are available on our LIMS Project Submission webpage.

DESCRIPTION PRICE PER
SAMPLE
Quantabio SparQ DNA library + 3 Gbp (10 M read pairs) $76
Quantabio SparQ DNA library + 6 Gbp (20 M read pairs) $102
Quantabio SparQ DNA library + 9 Gbp (30 M read pairs) $128
Quantabio SparQ DNA library + 12 Gbp (40 M read pairs) $154
Additional sequencing can be purchased in 3 Gbp increments $26 per 3 Gbp

The Quantabio SparQ DNA library prep is the standard shotgun DNA library preparation kit used by the Genomics Core.  This kit can be used with genomic and metagenomic DNA samples.  Low input and ChIP-seq library preparations are also possible with this method. 

The prices in the table below are for library preparation only.  The type and amount of sequencing will be selected separately.

DNA sample requirements are given on our Sample Requirements webpage.

QUANTITY

PRICE PER LIBRARY

≤ 12 samples $68
13 - 47 samples $57
≥ 48 samples $50

KAPA HyperPrep DNA libraries are only available for legacy projects that have previously used this prep method.  Contact the Genomics Core at gtsf@msu.edu to discuss project prior to submission.

The prices in the table below are for library preparation only.  The type and amount of sequencing will be selected separately.

QUANTITY PRICE PER LIBRARY
≤ 12 samples $93
13 - 47 samples $81
≥ 48 samples $74

The prices in the table below are for library preparation only.  The type and amount of sequencing will be selected separately.

DNA sample requirements are given on our Sample Requirements webpage.

QUANTITY PRICE PER LIBRARY
8 - 19 samples $223
≥ 20 samples $166

RNA-Seq Library Preparation Services

The Genomics Core is now offering Bundled Flat Rate Pricing for RNA-seq library preparation and sequencing on the AVITI.  This service allows researchers to pay a flat rate for library preparation and a set amount of sequencing for each sample.  The Watchmaker mRNA library prep method will be used for this service.

RNA sample requirements are given on our Sample Requirements webpage.

The Bundled Flat Rate Submission Forms are available on our LIMS Project  Submission webpage.

DESCRIPTION PRICE PER
SAMPLE
Watchmaker mRNA library + 25 M read pairs $131
Watchmaker mRNA library + 35 M read pairs $157
Watchmaker mRNA library + 45 M read pairs $183
Watchmaker mRNA library + 55 M read pairs $209

The Watchmaker mRNA library prep is the standard RNA-seq library preparation kit used by the Genomics Core.  This kit is appropriate for use with high-quality eukaryotic total RNA.

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

RNA sample requirements are given on our Sample Requirements webpage.

QUANTITY PRICE PER LIBRARY
≤ 12 samples $89
13 - 47 samples $73
≥ 48 samples $65

RNA-Seq libraries can be prerpared using ribosomal RNA depletion instead of polyA mRNA selection.  The Genomics Core uses Qiagen's QIASeq FastSelect Kits for this method.  FastSelect rRNA depletion are purchased by the researcher and provided to the Genomics Core.  Please contact the Genomics Core (gtsf@msu.edu) to discuss your project.

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

RNA sample requirements are given on our Sample Requirements webpage.

QUANTITY PRICE PER LIBRARY
≤ 12 samples $98
13 - 47 samples $82
≥ 48 samples $74

Quant-Seq generates highly strand-specific libraries of reads from the 3' end of each mRNA in your sample.

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

RNA sample requirements are given on our Sample Requirements webpage.

QUANTITY PRICE PER LIBRARY
≥ 72 samples $66

Library preparation of small RNAs (<200 nt), including microRNA (miRNA).

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

RNA sample requirements are given on our Sample Requirements webpage.

DESCRIPTION PRICE
Small RNA Library Preparation $145/library
Purification of Small RNA
Library Pool*
$67/pool

*Small RNA libraries almost always contain primer dimers because the desired insert is so small.  A purification is performed on the pool of libraries to reduce the amount of primer dimers.

KAPA mRNA HyperPrep libraries are only available for legacy projects that have previously used this prep method.  Contact the Genomics Core at gtsf@msu.edu to discuss project prior to submission.

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

QUANTITY PRICE PER LIBRARY
≤ 12 samples $98
13 - 47 samples $87
≥ 48 samples $80

KAPA HyperPrep RNA Library with FastSelect rRNA depletion are only available for legacy projects that have previously used this prep method.  The Genomics Core uses Qiagen's QIASeq FastSelect Kits for this method.  FastSelect rRNA depletion are purchased by the researcher and provided to the Genomics Core.  Contact the Genomics Core at gtsf@msu.edu to discuss project prior to submission.

The prices in the table below are for library preparation only.  The type and amount of sequencing is purchased separately.

QUANTITY PRICE PER LIBRARY
≤ 12 samples $107
13 - 47 samples $96
≥ 48 samples $89

Amplicon Library Preparation Services

16S V4 Amplicon library preparation uses the 515f/806r primer pairs.  For more information visit the 16S V4 section on the sample requirements webpage.

QUANTITY PRICE
24 - 380 samples

$5.50 per sample

plus $207 per plate

The Genomics Core will add barcodes to primary PCR products.  For more information and instructions regarding proper construction of primary PCR products visit the Amplicon Barcoding section on the sample requirements webpage.

QUANTITY PRICE
24 - 380 samples

$5.50 per sample

plus $207 per plate

10X Genomics Chromium Single Cell Library Preparation Services

A consultation with the Genomics Core is required prior to beginning experiment.  Contact us at gtsf@msu.edu to schedule consult.  Researcher is required to purchase 10X kit(s).

QUANTITY PRICE PER SAMPLE
1 sample $570
2 - 4 samples $337
5 - 8 samples $223

A consultation with the Genomics Core is required prior to beginning experiment.  Contact us at gtsf@msu.edu to schedule consult.  Researcher is required to purchase 10X kit(s).

QUANTITY PRICE PER SAMPLE
1 sample $508
2 - 4 samples $285
5 - 8 samples $177

A consultation with the Genomics Core is required prior to beginning experiment.  Contact us at gtsf@msu.edu to schedule consult.  Researcher is required to purchase 10X kit(s).

QUANTITY PRICE PER SAMPLE
1 sample $1,036
2 - 4 samples $641
5 - 8 samples $378

Short-Read Library/Pool Quality Control

DESCRIPTION PRICE
qPCR validation of library pool prepared by Genomics Core
(Excluding amplicon library pools)
$28
QC of amplicon library pool prepared by Genomics Core
(Includes fluorometric quantification, TapeStation analysis, and qPCR validation)
$68
QC of user-prepared library or pool
(Includes fluorometric quantification, TapeStation analysis, and qPCR validation)
$68

*NEW* AVITI Sequencing

The AVITI is the new sequencing instrument in the Genomics Core. The AVITI offers less expensive sequencing options, higher accuracy, and lower duplication rates than the NovaSeq 6000.  Sequencing options in the table below are available for purchase by the flow cell.  It is possible to purchase half of the AVITI 2x150 bp High Capacity Flow Cell.

READ FORMAT*

CAPACITY UNIT # READ PAIRS GBP PRICE

2x75 bp
OR
1x150 bp

Medium Flow Cell 500 million 75 $1,181
High Flow Cell 1 billion 150 $1,408
2x150 bp Low Flow Cell 250 million 75 $1,181
Medium Flow Cell 500 million 150 $1,408
High Half Flow Cell 500 million 150 $1,059
High Flow Cell 1 billion 300 $2,089
2x300 bp Medium Flow Cell 100 million 60 $2,089
High Flow Cell 300 million 180 $2,950

*The read format can be adjusted to accomodate 10X single cell libraries or other libraries with unusual read formats.  Please contact the Genomics Core at gtsf@msu.edu to discuss changes in read format prior to submission.

NovaSeq 6000 Sequencing

The NovaSeq 6000 sequencing options in the table below are available for purchase by the lane.

The NovaSeq 6000 will be retired in December 2024.

TYPE READ FORMAT UNIT # READS GBP PRICE
SP 1x100 bp Lane 362 M single reads 36 $1,729
SP 2x150 bp Lane 362 M read pairs 108 $2,463
S1 1x100 bp Lane 725 M single reads 73 $2,910
S1 2x150 bp Lane 725 M read pairs 218 $4,204
S4 2x150 bp Lane 2,250 M read pairs 675 $5,953
S4-shared 2x150 bp 1/10th Lane 200 M read pairs 60 $694

The NovaSeq 6000 sequencing options in the table below are available for purchase by the flow cell.

The NovaSeq 6000 will be retired in December 2024.

TYPE READ FORMAT UNIT # READS GBP PRICE
SP 1x100 bp Flow Cell 724 M single reads 72 $3,458
2x50 bp Flow Cell 724 M read pairs 72 $3,458
2x100 bp Flow Cell 724 M read pairs 144 $4,420
2x150 bp Flow Cell 724 M read pairs 216 $4,926
2x250 bp Flow Cell 724 M read pairs 362 $6,838
S1
1x100 bp Flow Cell 1,450 M single reads 146 $5,820
2x50 bp Flow Cell 1,450 M read pairs 146 $5,820
2x100 bp Flow Cell 1,450 M read pairs 292 $7,486
2x150 bp Flow Cell 1,450 M read pairs 436 $8,408
S2
1x100 bp Flow Cell 3,700 M single reads 370 $10,366
2x50 bp Flow Cell 3,700 M read pairs 370 $10,366
2x100 bp Flow Cell 3,700 M read pairs 740 $13,258
2x150 bp Flow Cell 3,700 M read pairs 1,110 $14,730
S4 2x100 bp Flow Cell 9,000 million read pairs 1,800 $20,476
2x150 bp Flow Cell 9,000 million read pairs 2,700 $23,812

MiSeq Sequencing

The MiSeq v2 chemistry is recommended for amplicon sequencing.  Note that output is expected to decrease by 30 - 40% when sequencing amplicon libraries or other low diversity libraries.

FLOW CELL TYPE READ FORMAT # READ PAIRS GBP PRICE

v2 Standard

2x150 bp
(300 cycle)

8 - 10 M for amplicon libraries

12 - 15 M for standard DNA and RNA libraries

3.6-4.5 $1,610

2x250 bp
(500 cycle)

8 - 10 M for amplicon libraries

12 - 15 M for standard DNA and RNA libraries

6.0-7.5 $1,751
v2 Micro 2x150 bp
(300 cycle)
4 M for standard DNA and RNA libraries 1.2 $802
v2 Nano 2x150 bp
(300 cycle)
1 M for standard DNA and RNA libraries 0.3 $604
2x250 bp
(500 cycle)
1 M for standard DNA and RNA libraries 0.5 $748

The MiSeq v3 is not recommended for sequencing amplicon libraries or other low diversity libraries.

FLOW CELL TYPE READ FORMAT # READ PAIRS GBP PRICE
v3

1x150 bp
OR
2x75 bp
(150 cycle)

22 - 25 M for standard DNA and RNA libraries 3.3-3.8 $1,375
2x300 bp
(600 cycle)
22 - 25 M for standard DNA and RNA libraries 13-15 $2,354

Library/pool Size-Selection

Select-a-Size (Zymo part number D4080) can be used to select for fragments ≥ 300 bp or ≥ 200 bp.  This size selection method is not offered for individual samples.  It is used by the Genomics Core to reduce the presence of barcoded primer dimers in amplicon libraries.  Researchers should contact the Genomics Core (gtsf@msu.edu) before submission of user-prepared libraries or pool(s) if they feel that size selection is needed.

DESCRIPTION PRICE
Size selection for fragments
≥ 300 bp or ≥ 200 bp

$18 per library or pool

 

The Sage Blue Pippin Size can be used for size selection of completed libraries or pools.  The Genomics Core only offers one cassette, with this cassette the size range for selection is 100 - 600 bp.  This size selection method is not offered for individual samples.  It is used by the Genomics Core to reduce the presence of primer dimers in completed libraries.  Researchers should contact the Genomics Core (gtsf@msu.edu) before submission of user-prepared libraries or pool(s) if they feel that size selection is needed.

DESCRIPTION PRICE

Blue Pippin Size Selection
(100 - 600 bp size range only)

Libraries must be similar size
and require the same size
selection settings.

$124 for selection of
1 to 4 libraries at one time

A magnetic bead cleanup can be used to remove small contaminating fragements (i.e. primer dimers) from completed libraries or pools.  This size selection method is not offered for individual samples.  Researchers should contact the Genomics Core (gtsf@msu.edu) before submission of user-prepared libraries or pool(s) if they feel that a bead cleanup is needed.

DESCRIPTION PRICE
Magnetic Bead Cleanup

$35 per library or pool

 

Long-read Library Preparation & Sequencing (Oxford Nanopore)

See our Oxford Nanopore Technology page and our Nanopore Sample Requirements page for more information.

Oxford Nanopore Submission Form

DESCRIPTION

PRICE PER LIBRARY

Ligation Sequencing Kit
(LSK114)
$787
Ligation Sequencing with Native Barcoding Kit
(NBD114.24)
$690 + $144/barcode
(up to 24 samples)
Rapid Barcoding Kit
(RBK114.24)
$472
(up to 24 samples)
Direct RNA Sequencing Kit
(RNA004)
$559
cDNA-PCR Sequencing Kit
(PCS109/PSC111)
$616
cDNA-PCR Sequencing Kit with Barcoding
(PCB109/PCB111.24)
$445 + $114/barcode
(up to 24 samples)

See our Oxford Nanopore Technology page and our Nanopore Sample Requirements page for more information.

Oxford Nanopore Submission Form

DESCRIPTION

PRICE

GridION R10.4.1 $899
PromethION R10.4.1 $1,095

 

Other Services

Sanger Sequencing

For sample requirements, see our Sanger Sequencing Sample Requirements webpage.

The Sanger Sequencing Submission Form and Custom Naming Template can be downloaded from our Forms webpage.

DESCRIPTION

PRICE PER
REACTION

Low throughput standard reaction
(≤ 8 samples in individual tubes)
$7
Medium throughput standard reaction
(9 - 16 samples in 8-strip PCR tubes or 96-well plate)
$6
High throughput standard reaction
(≥ 17 samples in 96-well PCR plate)
$3.50
High throughput, low cost standard reaction
Researcher adds primer, no reruns
(≥ 48 samples in 96-well PCR plate)
$2.75

 

The 1st tier reaction is for samples with high GC content and plasmids > 10 kb.  If you have questions about the 1st Tier reaction, please contact the Genomics Core at gtsf@msu.edu.

For sample requirements, see our Sanger Sequencing Sample Requirements webpage.

The Sanger Sequencing Submission Form and Custom Naming Template can be downloaded from our Forms webpage.

DESCRIPTION

PRICE PER
REACTION

Low throughput 1st tier reaction
(≤ 8 samples in individual tubes)
$8
Medium throughput 1st tier reaction
(9 - 16 samples in 8-strip PCR tubes or 96-well plate)
$7
High throughput 1st tier reaction
(≥ 17 samples in 96-well PCR plate)
$4.50

GeneScan/Fragment Analysis

GeneScan/Fragment analysis services are available for the size standards below.  Analysis is run on the ABI 3730XL instrument.

DESCRIPTION

PRICE PER
SAMPLE

GeneScan 500 ROX dye Size Standard

$2.25
($50 minimum fee)

GeneScan 500 LIZ dye Size Standard

$2.25
($50 minimum fee)

GeneScan 400HD ROX dye Size Standard

$2.25
($50 minimum fee)

MapMarker X-Rhodamine Labeled 50-1000bp

$1.80
($50 minimum fee)

GeneScan 1200 LIZ dye Size Standard

$2.40
($50 minimum fee)

QuantStudio 7 Flex qPCR

Researcher submits fully assembled reactions in 96-well or 384-well plate(s).  The Genomics Core does not assemble reactions for researchers.

The qPCR submission form can be downloaded from our Forms webpage.

Please see our qPCR webpage for more information.

DESCRIPTION

PRICE

One (1) 96-well plate $59
Two (2) 96-well plates  $69
Three (3) 96-well plates $80
Four (4) 96-well plates $90
One (1) 384-well plate $38
Dissociation Curve $8.50

The Genomics Core allows researchers to purchase 384-well plates and optical film for submissions that will be made to the Genomics Core.

ITEM

PRICE

384-well optical plate, each $5
Optical sealing film, each $3

Covaris Sonicator (Self-Service Equipment)

Researchers that bring their own covaris tubes for use with the M220 Sonicator are charged sample shearing fee.

DESCRIPTION

PRICE PER
SAMPLE

Sample Shearing $2

Visit the Common Use Equipment webpage for instructions to reserve the Covaris.

The Genomics Core has two types of Covaris tubes available for purchase.

DESCRIPTION

PRICE PER
TUBE

Small Tube (130 µl tube, p/n 520045) $6.50
Large Tube (1 ml tube, p/n 520135) $7

Visit the Common Use Equipment webpage for instructions to reserve the Covaris.

NanoString nCounter

Researchers that are interested in the NanoString should contact the Genomics Core at gtsf@msu.edu to discuss instrument availability.

Price does not include reagents from NanoString. The listed prices are for sample processing only.  

DESCRIPTION

PRICE

mRNA assay with Gene Expression Panel
(Custom CodeSet or Elements TagSet)
$247/12 sample cassette
miRNA assay with NanoString miRNA Panel $251/12 sample cassette
Multiplexed mRNA assay with nCounter PlexSet $356/96 samples in a 12 well cassette