TapeStation Sample Requirements
The Genomics Core has an Agilent 4200 TapeStation to provide analysis of total RNA, prepared libraries, dsDNA PCR products, fragmented DNA, and high molecular weight genomic DNA.
Sample concentrations must be measured by fluorometric method, such as Qubit or PicoGreen, and diluted appropriately according to the table below.
TapeStation analysis pricing is described on our pricing page page.
Instructions for submitting samples are at the bottom of the page.
TapeStation Sample Requirements
| TapeStation Assay | Use | Acceptable Concentration Range (ng/µl) | Minimum Volume (µl) |
|---|---|---|---|
| High Sensitivity RNA | For analysis of total RNA | 1 - 5 | 10 |
|
High Sensitivity D1000 (sizing range 35 - 1,000 bp) |
For analysis of prepared libraries, dsDNA PCR products, or fragmented DNA. |
0.5 - 1 | 5 |
|
High Sensitivity D5000 (sizing range 100 - 5,000 bp) |
For analysis of prepared libraries, dsDNA PCR products, or fragmented DNA. | 0.5 - 1 | 5 |
| Genomic DNA (sizing range 200 - 60,000 bp) |
For analysis of high molecular weight genomic DNA. | 25 - 50 | 5 |
Submitting Samples for Analysis
For 15 samples or less, samples can be submitted in PCR strip-tubes or 1.5 ml microcentrifuge tubes. For 16 or more samples, samples must be submitted in a 96-well PCR-type plate, filled by column. All tubes and plates must be clearly labeled. If you cannot clearly label the tubes or plate, you must place them in a secondary container that is properly labeled.
Samples must be at the appropriate concentration and at the correct volume as specified in table above. The Genomics Core will not quantify or dilute samples submitted for analysis. Samples that are not submitted as specified will not be processed. Samples submitted for analysis will not be returned to researcher and will be discarded after 2 weeks.
Researchers will pre-register their samples in the LIMS system. See our LIMS Project Submission webpage for information about requesting a LabLink account, creating a project, and to download submission forms.
Once the project is registered in the LIMS, samples will be delivered to the Genomics Core along with the signed LIMS submission sheet. Place samples in the -20 freezer (bin labeled "TapeStation") in the hallway outside of the Genomics Core (S-18 Plant Biology). Place the LIMS submission form in the basket labeled "TapeStation" that is attached to the outside of the freezer. Results are typically available on Genomics Depot within 48 business hours.