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Mass Spectrometry and Metabolomics Core

Mass Spectrometry and Metabolomics Core

The RTSF Mass Spectrometry and Metabolomics Core is an ‘open access’ resource where MSU researchers and Core staff perform chemical analyses for small molecules and intact macromolecules. The Core features 9 autosampler-equipped mass spectrometers that collectively provide GC/MS, GC/MS/MS, LC/MS/MS, and accurate mass (high resolution). Analyses performed here typically involve compound identification, quantification, and statistical analysis. Core staff are available to provide expert consultation regarding method development and data interpretation. Students and other researchers are encouraged to become certified users of the instruments; training includes discussions of theory and operation of the Core instrumentation; following training, users are provided 24/7 access to the instruments. Methods currently running include non-targeted metabolite profiling (metabolomics) and high-throughput profiling and quantification of amino acids, phytohormones, nucleotides signaling oxylipins, structural lipids, and central and specialized metabolites. The Core performs analyses of samples from more than 100 institutions from across North America.

Equipment

LC/MS/MS (tandem quadrupole)

  • Waters TQ-D UPLC/MS/MS
  • Waters Xevo TQ-S UPLC/MS/MS
  • Waters Xevo TQ-XS UPLC/MS/MS
  • Waters Xevo TQ-S Micro UPLC/MS/MS

LC/MS/MS (High Mass Resolution Systems)

  • Quadrupole/Time-of-Flight systems
    • Waters Xevo G2-XS UPLC/MS/MS (2 instruments)
  • Quadrupole/Orbitrap system
    • Thermo Q-Exactive UPLC/MS/MS

GC/MS

  • Agilent 5975 GC/single quadrupole MS
  • Agilent 7010 GC/triple quadrupole MS/MS

Submission

The Core is, if time allows, accessible to academic investigators from any institution. Samples will be accepted provided they are appropriate for current Core capabilities. Access is also granted to occasional industrial clients on a time-available basis. Prior to sending samples, investigators are recommended to contact the Core staff to discuss the required analysis. This is necessary to insure that the most efficient and cost-effective analytical methods are employed. Samples are normally analyzed in the order of their receipt, but special arrangements can be made for unstable samples. A sample submission form should accompany each set of samples. The information requested on this form should be filled out completely to assure that the analyses can be carried out properly. Missing information from the form (e.g., sample solubility, stability, molecular formula, if known) will result in delays in the requested analyses.

Mass Spectrometry and Metabolomics Core billing codes and rates