Mass Spectrometry and Metabolomics Core
The RTSF Mass Spectrometry and Metabolomics Core is an ‘open access’ resource where MSU researchers and Core staff perform chemical analyses for small molecules and intact macromolecules. The Core features 12 autosampler-equipped mass spectrometers that collectively provide GC-MS, LC-MS/MS, accurate mass (high resolution), and MALDI-TOF capabilities. Analyses performed here typically involve compound identification, quantification, and statistical analysis. Core staff are available to provide expert consultation regarding method development and data interpretation. Students and other researchers are encouraged to become certified users of the instruments; training includes discussions of theory and operation of the Core instrumentation; following training, users are provided 24/7 access to the instruments. Methods currently running include nontarget metabolite profiling (metabolomics) and high-throughput profiling and quantification of amino acids, phytohormones, nucleotides signaling oxylipins, structural lipids, and central and specialized metabolites. Capabilities for MALDI-TOF tissue and surface imaging have also been developed. The Core performs analyses of samples from more than 100 institutions from across North America.
The Core is, if time allows, accessible to academic investigators from any institution. Samples will be accepted provided they are appropriate for current Core capabilities. Access is also granted to occasional industrial clients on a time-available basis. Prior to sending samples, investigators are recommended to contact the Core staff to discuss the required analysis. This is necessary to insure that the most efficient and cost-effective analytical methods are employed. Samples are normally analyzed in the order of their receipt, but special arrangements can be made for unstable samples. A sample submission form should accompany each set of samples. The information requested on this form should be filled out completely to assure that the analyses can be carried out properly. Missing information from the form (e.g., sample solubility, stability, molecular formula, if known) will result in delays in the requested analyses.
The Core is licensed to handle samples containing 3H, 14C and 32P and 35S isotopes. However, to meet NRC regulations a different sample submission protocol through the MSU Office of Radiation, Chemical and Biological Safetyis necessary (see below). Michigan State University requires that this mailing procedure be followed even for trace levels of radioactive material.
Submitting Radioactive Samples
All radioactive samples must be initially processed by the MSU Office of Radiation, Chemical and Biological Safety (ORCBS) BEFORE they can be received by the MSU Mass Spectrometry and Metabolomics Core.