Resource Information for Grant Applications

The MSU Genomics Core provides many types of sequencing services to both MSU researchers and the wider research community.  The Genomics Core also provides instrumentation for SNP profiling, fragment analysis genotyping and other nucleic acid QC services.  Equipment within the Core includes one Element Biosciences AVITI24 and one Element Biosciences AVITI for next-generation sequencing.  A 10x Genomics Chromium X is used to generate single cell sequencing libraries.  A 10x Genomics CytAssist is used to prepare Visium spatial transcriptome libraries.  Three Oxford Nanopore PromethION P2 solo instruments are available for long-read DNA, cDNA and direct RNA sequencing.  An ABI 3730xl 96-capillary DNA sequencer performs traditional Sanger sequencing, fragment analysis and SNP sequencing.  Size-specific shearing of nucleic acids is achieved with a Covaris M220 Sonicator.  Sequencing library size-selection is performed with a Sage Science Pippin Prep.  Two Agilent 4200 TapeStations are used for RNA and DNA quality and size analysis.  Fluorescence-based nucleic acid quantification is performed on an Invitrogen Qubit and Qubit Flex devices.