Resource Information for Grant Applications
The MSU Genomics Core provides many types of sequencing services to both MSU researchers and the wider research community. The Genomics Core also provides instrumentation for SNP profiling, fragment analysis genotyping, Quantitative Real Time PCR and other nucleic acid QC services. Equipment within the Core includes an Element Biosciences AVITI, an Illumina NovaSeq 6000, and two Illumina MiSeqs for next-generation sequencing. A 10X Chromium Controller is used to generate single cell sequencing libraries. Two Oxford Nanopore PromethION P2 solo instrument and a GridION instrument are available for long-read DNA, cDNA and direct RNA sequencing. An ABI 3730xl 96-capillary DNA sequencer performs traditional Sanger sequencing, fragment analysis and SNP sequencing. An ABI QuantStudio 7 Flex is used for qPCR analysis. Digital gene expression analysis is performed on a NanoString nCounter. Size-specific shearing of nucleic acids is achieved with a Covaris M220 Sonicator. An Agilent 4200 TapeStation is used for RNA and DNA quality and size analysis. Fluorescence-based nucleic acid quantification is performed on an Invitrogen Qubit and Qubit Flex devices.